ssr_primer
Primer Details for TRINITY_DN13233_c2_g2_i4 |
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| (TTG)9gtgttgaagataagctttctccatctgtaacttatggttgttcatattgttgtcttt(TCC)7: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGTGATGTTGTTGGACCCCT | GGAGGAGGAGGAGGAGGAAA | Start: 25 |
| Tm | 58.851 | 59.66 | End: 150 |
| Size | 20 | 20 | 126 |
| Primer 2 details |
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| Sequence | AGTGATGTTGTTGGACCCCT | AAAGGAGGAGGAGGAGGAGG | Start: 25 |
| Tm | 58.851 | 59.658 | End: 156 |
| Size | 20 | 20 | 132 |
| Primer 3 details |
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| Sequence | TTTCCTCCTCCTCCTCCTCC | TGCTGAGTTATGGGGAAAGCA | Start: 131 |
| Tm | 59.66 | 59.646 | End: 259 |
| Size | 20 | 21 | 129 |
| Primer 4 details |
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| Sequence | CCTCCTCCTCCTCCTCCTTT | TGCTGAGTTATGGGGAAAGCA | Start: 137 |
| Tm | 59.658 | 59.646 | End: 259 |
| Size | 20 | 21 | 123 |
| Primer 5 details |
|---|
| Sequence | AGTGATGTTGTTGGACCCCT | AGGAGGAGGAGGAGGAGGAA | Start: 25 |
| Tm | 58.851 | 60.556 | End: 151 |
| Size | 20 | 20 | 127 |
(GAGAAA)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGTGATGTTGTTGGACCCCT | GGAGGAGGAGGAGGAGGAAA | Start: 25 |
| Tm | 58.851 | 59.66 | End: 150 |
| Size | 20 | 20 | 126 |
| Primer 2 details |
|---|
| Sequence | AGTGATGTTGTTGGACCCCT | AAAGGAGGAGGAGGAGGAGG | Start: 25 |
| Tm | 58.851 | 59.658 | End: 156 |
| Size | 20 | 20 | 132 |
| Primer 3 details |
|---|
| Sequence | TTTCCTCCTCCTCCTCCTCC | TGCTGAGTTATGGGGAAAGCA | Start: 131 |
| Tm | 59.66 | 59.646 | End: 259 |
| Size | 20 | 21 | 129 |
| Primer 4 details |
|---|
| Sequence | CCTCCTCCTCCTCCTCCTTT | TGCTGAGTTATGGGGAAAGCA | Start: 137 |
| Tm | 59.658 | 59.646 | End: 259 |
| Size | 20 | 21 | 123 |
| Primer 5 details |
|---|
| Sequence | AGTGATGTTGTTGGACCCCT | AGGAGGAGGAGGAGGAGGAA | Start: 25 |
| Tm | 58.851 | 60.556 | End: 151 |
| Size | 20 | 20 | 127 |
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