ssr_primer
Primer Details for TRINITY_DN13225_c4_g3_i2 |
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| (A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCACCAAAGGATCCGCTTCC | AGTTCGCTGGTTTTGGTGGA | Start: 295 |
| Tm | 60.035 | 60.107 | End: 442 |
| Size | 20 | 20 | 148 |
| Primer 2 details |
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| Sequence | ATCCGCTTCCAATCTTCCGG | AGTTCGCTGGTTTTGGTGGA | Start: 305 |
| Tm | 60.179 | 60.107 | End: 442 |
| Size | 20 | 20 | 138 |
| Primer 3 details |
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| Sequence | CACCAAAGGATCCGCTTCCA | AGTTCGCTGGTTTTGGTGGA | Start: 296 |
| Tm | 60.322 | 60.107 | End: 442 |
| Size | 20 | 20 | 147 |
| Primer 4 details |
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| Sequence | CTTCCAATCTTCCGGCCCAA | AGTTCGCTGGTTTTGGTGGA | Start: 310 |
| Tm | 60.323 | 60.107 | End: 442 |
| Size | 20 | 20 | 133 |
| Primer 5 details |
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| Sequence | ACAGCCACTTCCTCCTCAGT | GGAAGCGGATCCTTTGGTGA | Start: 97 |
| Tm | 60.473 | 60.035 | End: 314 |
| Size | 20 | 20 | 218 |
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