ssr_primer
Primer Details for TRINITY_DN13225_c4_g1_i3 |
|---|
| (TCC)6: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGATTCCTTCTTCACCGCCA | CGGGATCCACAAACTCACCA | Start: 3124 |
| Tm | 60.035 | 59.964 | End: 3402 |
| Size | 20 | 20 | 279 |
| Primer 2 details |
|---|
| Sequence | CTCAACACGGGTGATGGGAA | TGTCTACTGGTTTTCGGCCC | Start: 5130 |
| Tm | 59.964 | 59.964 | End: 5266 |
| Size | 20 | 20 | 137 |
| Primer 3 details |
|---|
| Sequence | GGATTCCTTCTTCACCGCCA | TGATGGTGTGAAGACTGGGC | Start: 3124 |
| Tm | 60.035 | 59.963 | End: 3368 |
| Size | 20 | 20 | 245 |
| Primer 4 details |
|---|
| Sequence | TGGAAGTGCGGGTGATCTTC | CGGGATCCACAAACTCACCA | Start: 3173 |
| Tm | 60.036 | 59.964 | End: 3402 |
| Size | 20 | 20 | 230 |
| Primer 5 details |
|---|
| Sequence | GGGGGATTTGGCCGAGTTTA | CGCCCTTCAATGCATATGCC | Start: 3693 |
| Tm | 60.034 | 60.039 | End: 3868 |
| Size | 20 | 20 | 176 |
(T)16: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGATTCCTTCTTCACCGCCA | CGGGATCCACAAACTCACCA | Start: 3124 |
| Tm | 60.035 | 59.964 | End: 3402 |
| Size | 20 | 20 | 279 |
| Primer 2 details |
|---|
| Sequence | CTCAACACGGGTGATGGGAA | TGTCTACTGGTTTTCGGCCC | Start: 5130 |
| Tm | 59.964 | 59.964 | End: 5266 |
| Size | 20 | 20 | 137 |
| Primer 3 details |
|---|
| Sequence | GGATTCCTTCTTCACCGCCA | TGATGGTGTGAAGACTGGGC | Start: 3124 |
| Tm | 60.035 | 59.963 | End: 3368 |
| Size | 20 | 20 | 245 |
| Primer 4 details |
|---|
| Sequence | TGGAAGTGCGGGTGATCTTC | CGGGATCCACAAACTCACCA | Start: 3173 |
| Tm | 60.036 | 59.964 | End: 3402 |
| Size | 20 | 20 | 230 |
| Primer 5 details |
|---|
| Sequence | GGGGGATTTGGCCGAGTTTA | CGCCCTTCAATGCATATGCC | Start: 3693 |
| Tm | 60.034 | 60.039 | End: 3868 |
| Size | 20 | 20 | 176 |
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