ssr_primer
Primer Details for TRINITY_DN13225_c3_g2_i3 |
|---|
| (A)11: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAAGCTCGAGCCTCACCTTC | GCCAATGCCCCATTTCCATC | Start: 870 |
| Tm | 60.037 | 59.893 | End: 1146 |
| Size | 20 | 20 | 277 |
| Primer 2 details |
|---|
| Sequence | AAACGTGGATGTGCAGGGAT | GGAATTCCTCTGCGCCTTCT | Start: 573 |
| Tm | 59.962 | 60.107 | End: 838 |
| Size | 20 | 20 | 266 |
| Primer 3 details |
|---|
| Sequence | AAACGTGGATGTGCAGGGAT | AGGAATTCCTCTGCGCCTTC | Start: 573 |
| Tm | 59.962 | 60.107 | End: 839 |
| Size | 20 | 20 | 267 |
| Primer 4 details |
|---|
| Sequence | AAAGCTCGAGCCTCACCTTC | TGCTGCCTTTCTCTTGCTCA | Start: 870 |
| Tm | 60.037 | 59.891 | End: 1062 |
| Size | 20 | 20 | 193 |
| Primer 5 details |
|---|
| Sequence | AACTAGACAGTAACGCCGCC | ATCCCTGCACATCCACGTTT | Start: 429 |
| Tm | 60.109 | 59.962 | End: 592 |
| Size | 20 | 20 | 164 |
(AT)8: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAAGCTCGAGCCTCACCTTC | GCCAATGCCCCATTTCCATC | Start: 870 |
| Tm | 60.037 | 59.893 | End: 1146 |
| Size | 20 | 20 | 277 |
| Primer 2 details |
|---|
| Sequence | AAACGTGGATGTGCAGGGAT | GGAATTCCTCTGCGCCTTCT | Start: 573 |
| Tm | 59.962 | 60.107 | End: 838 |
| Size | 20 | 20 | 266 |
| Primer 3 details |
|---|
| Sequence | AAACGTGGATGTGCAGGGAT | AGGAATTCCTCTGCGCCTTC | Start: 573 |
| Tm | 59.962 | 60.107 | End: 839 |
| Size | 20 | 20 | 267 |
| Primer 4 details |
|---|
| Sequence | AAAGCTCGAGCCTCACCTTC | TGCTGCCTTTCTCTTGCTCA | Start: 870 |
| Tm | 60.037 | 59.891 | End: 1062 |
| Size | 20 | 20 | 193 |
| Primer 5 details |
|---|
| Sequence | AACTAGACAGTAACGCCGCC | ATCCCTGCACATCCACGTTT | Start: 429 |
| Tm | 60.109 | 59.962 | End: 592 |
| Size | 20 | 20 | 164 |
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