ssr_primer
Primer Details for TRINITY_DN13222_c2_g1_i1 |
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| (A)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TAACCGAGTTTGGGAGGCAC | TGGCCACGTTTGTTCTAGCA | Start: 243 |
| Tm | 59.964 | 60.179 | End: 487 |
| Size | 20 | 20 | 245 |
| Primer 2 details |
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| Sequence | ATCTGCAGCTTTGCTCTCGT | TTGGGGATGTTGGCGAAACT | Start: 2222 |
| Tm | 60.037 | 60.179 | End: 2499 |
| Size | 20 | 20 | 278 |
| Primer 3 details |
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| Sequence | AGTTTCGCCAACATCCCCAA | GCAGGCCAATTCGTTCGTTT | Start: 2480 |
| Tm | 60.179 | 60.04 | End: 2675 |
| Size | 20 | 20 | 196 |
| Primer 4 details |
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| Sequence | TAACCGAGTTTGGGAGGCAC | AAACGTCTGGCCCAGTCTTT | Start: 243 |
| Tm | 59.964 | 59.817 | End: 426 |
| Size | 20 | 20 | 184 |
| Primer 5 details |
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| Sequence | ATCTGCAGCTTTGCTCTCGT | CTTGGGGATGTTGGCGAAAC | Start: 2222 |
| Tm | 60.037 | 59.756 | End: 2500 |
| Size | 20 | 20 | 279 |
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