ssr_primer
Primer Details for TRINITY_DN13219_c3_g1_i19 |
|---|
| (T)11: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCCTGTTCATCGTCGCATTC | TGGGGAAGTGGGTTCGAATG | Start: 10 |
| Tm | 59.973 | 59.962 | End: 214 |
| Size | 20 | 20 | 205 |
| Primer 2 details |
|---|
| Sequence | ACCCTTCTAATCCCCTCCCC | GAGGAGGTGGCATATTCCGG | Start: 66 |
| Tm | 60.029 | 59.965 | End: 319 |
| Size | 20 | 20 | 254 |
| Primer 3 details |
|---|
| Sequence | ACCCTTCTAATCCCCTCCCC | GGAGGAGGTGGCATATTCCG | Start: 66 |
| Tm | 60.029 | 59.965 | End: 320 |
| Size | 20 | 20 | 255 |
| Primer 4 details |
|---|
| Sequence | ACCCTTCTAATCCCCTCCCC | CGGAGGAGGTGGCATATTCC | Start: 66 |
| Tm | 60.029 | 59.965 | End: 321 |
| Size | 20 | 20 | 256 |
| Primer 5 details |
|---|
| Sequence | CCTTCTAATCCCCTCCCCCA | GAGGAGGTGGCATATTCCGG | Start: 68 |
| Tm | 60.029 | 59.965 | End: 319 |
| Size | 20 | 20 | 252 |
(T)15: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCCTGTTCATCGTCGCATTC | TGGGGAAGTGGGTTCGAATG | Start: 10 |
| Tm | 59.973 | 59.962 | End: 214 |
| Size | 20 | 20 | 205 |
| Primer 2 details |
|---|
| Sequence | ACCCTTCTAATCCCCTCCCC | GAGGAGGTGGCATATTCCGG | Start: 66 |
| Tm | 60.029 | 59.965 | End: 319 |
| Size | 20 | 20 | 254 |
| Primer 3 details |
|---|
| Sequence | ACCCTTCTAATCCCCTCCCC | GGAGGAGGTGGCATATTCCG | Start: 66 |
| Tm | 60.029 | 59.965 | End: 320 |
| Size | 20 | 20 | 255 |
| Primer 4 details |
|---|
| Sequence | ACCCTTCTAATCCCCTCCCC | CGGAGGAGGTGGCATATTCC | Start: 66 |
| Tm | 60.029 | 59.965 | End: 321 |
| Size | 20 | 20 | 256 |
| Primer 5 details |
|---|
| Sequence | CCTTCTAATCCCCTCCCCCA | GAGGAGGTGGCATATTCCGG | Start: 68 |
| Tm | 60.029 | 59.965 | End: 319 |
| Size | 20 | 20 | 252 |
(A)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCCTGTTCATCGTCGCATTC | TGGGGAAGTGGGTTCGAATG | Start: 10 |
| Tm | 59.973 | 59.962 | End: 214 |
| Size | 20 | 20 | 205 |
| Primer 2 details |
|---|
| Sequence | ACCCTTCTAATCCCCTCCCC | GAGGAGGTGGCATATTCCGG | Start: 66 |
| Tm | 60.029 | 59.965 | End: 319 |
| Size | 20 | 20 | 254 |
| Primer 3 details |
|---|
| Sequence | ACCCTTCTAATCCCCTCCCC | GGAGGAGGTGGCATATTCCG | Start: 66 |
| Tm | 60.029 | 59.965 | End: 320 |
| Size | 20 | 20 | 255 |
| Primer 4 details |
|---|
| Sequence | ACCCTTCTAATCCCCTCCCC | CGGAGGAGGTGGCATATTCC | Start: 66 |
| Tm | 60.029 | 59.965 | End: 321 |
| Size | 20 | 20 | 256 |
| Primer 5 details |
|---|
| Sequence | CCTTCTAATCCCCTCCCCCA | GAGGAGGTGGCATATTCCGG | Start: 68 |
| Tm | 60.029 | 59.965 | End: 319 |
| Size | 20 | 20 | 252 |
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