ssr_primer
Primer Details for TRINITY_DN13217_c2_g1_i1 |
|---|
| (AT)8: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCTCTTGAGTTTCGTAACGGT | TCACGGGAGATGTCAGGAGA | Start: 133 |
| Tm | 59.375 | 59.668 | End: 314 |
| Size | 22 | 20 | 182 |
| Primer 2 details |
|---|
| Sequence | TCCCCAAGCAAATGTCTTCA | TCACGGGAGATGTCAGGAGA | Start: 105 |
| Tm | 57.326 | 59.668 | End: 314 |
| Size | 20 | 20 | 210 |
| Primer 3 details |
|---|
| Sequence | AGGCCTAGAATGCTTCCTCA | GTGTGGAAGACTATCGCACCT | Start: 241 |
| Tm | 58.115 | 59.795 | End: 358 |
| Size | 20 | 21 | 118 |
| Primer 4 details |
|---|
| Sequence | AGGCCTAGAATGCTTCCTCA | AGGTGTGGAAGACTATCGCAC | Start: 241 |
| Tm | 58.115 | 59.795 | End: 360 |
| Size | 20 | 21 | 120 |
| Primer 5 details |
|---|
| Sequence | CCCCAAGCAAATGTCTTCATCA | TCACGGGAGATGTCAGGAGA | Start: 106 |
| Tm | 59.173 | 59.668 | End: 314 |
| Size | 22 | 20 | 209 |
(T)10ggtgttttctcttcattctatcacaatagtattatcttctttggtttttctcaatctctcatctcatgaagattaattttttttt(A)11ggaaaaaagt(A)16: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCTCTTGAGTTTCGTAACGGT | TCACGGGAGATGTCAGGAGA | Start: 133 |
| Tm | 59.375 | 59.668 | End: 314 |
| Size | 22 | 20 | 182 |
| Primer 2 details |
|---|
| Sequence | TCCCCAAGCAAATGTCTTCA | TCACGGGAGATGTCAGGAGA | Start: 105 |
| Tm | 57.326 | 59.668 | End: 314 |
| Size | 20 | 20 | 210 |
| Primer 3 details |
|---|
| Sequence | AGGCCTAGAATGCTTCCTCA | GTGTGGAAGACTATCGCACCT | Start: 241 |
| Tm | 58.115 | 59.795 | End: 358 |
| Size | 20 | 21 | 118 |
| Primer 4 details |
|---|
| Sequence | AGGCCTAGAATGCTTCCTCA | AGGTGTGGAAGACTATCGCAC | Start: 241 |
| Tm | 58.115 | 59.795 | End: 360 |
| Size | 20 | 21 | 120 |
| Primer 5 details |
|---|
| Sequence | CCCCAAGCAAATGTCTTCATCA | TCACGGGAGATGTCAGGAGA | Start: 106 |
| Tm | 59.173 | 59.668 | End: 314 |
| Size | 22 | 20 | 209 |
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