ssr_primer
Primer Details for TRINITY_DN13217_c1_g1_i2 |
|---|
| (A)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AACCAATTTCAAAGGCGGGC | GGTTCCGAATGGGCAAATCG | Start: 1302 |
| Tm | 59.965 | 59.899 | End: 1542 |
| Size | 20 | 20 | 241 |
| Primer 2 details |
|---|
| Sequence | CGATTTGCCCATTCGGAACC | AACACTCAGCGGATTGAGCA | Start: 1523 |
| Tm | 59.899 | 59.965 | End: 1706 |
| Size | 20 | 20 | 184 |
| Primer 3 details |
|---|
| Sequence | AACCAATTTCAAAGGCGGGC | TACGATGGTTCCGAATGGGC | Start: 1302 |
| Tm | 59.965 | 60.179 | End: 1548 |
| Size | 20 | 20 | 247 |
| Primer 4 details |
|---|
| Sequence | GCCCATTCGGAACCATCGTA | AACACTCAGCGGATTGAGCA | Start: 1529 |
| Tm | 60.179 | 59.965 | End: 1706 |
| Size | 20 | 20 | 178 |
| Primer 5 details |
|---|
| Sequence | TCAACATCCATCGACCTCGC | AACACTCAGCGGATTGAGCA | Start: 1591 |
| Tm | 60.179 | 59.965 | End: 1706 |
| Size | 20 | 20 | 116 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AACCAATTTCAAAGGCGGGC | GGTTCCGAATGGGCAAATCG | Start: 1302 |
| Tm | 59.965 | 59.899 | End: 1542 |
| Size | 20 | 20 | 241 |
| Primer 2 details |
|---|
| Sequence | CGATTTGCCCATTCGGAACC | AACACTCAGCGGATTGAGCA | Start: 1523 |
| Tm | 59.899 | 59.965 | End: 1706 |
| Size | 20 | 20 | 184 |
| Primer 3 details |
|---|
| Sequence | AACCAATTTCAAAGGCGGGC | TACGATGGTTCCGAATGGGC | Start: 1302 |
| Tm | 59.965 | 60.179 | End: 1548 |
| Size | 20 | 20 | 247 |
| Primer 4 details |
|---|
| Sequence | GCCCATTCGGAACCATCGTA | AACACTCAGCGGATTGAGCA | Start: 1529 |
| Tm | 60.179 | 59.965 | End: 1706 |
| Size | 20 | 20 | 178 |
| Primer 5 details |
|---|
| Sequence | TCAACATCCATCGACCTCGC | AACACTCAGCGGATTGAGCA | Start: 1591 |
| Tm | 60.179 | 59.965 | End: 1706 |
| Size | 20 | 20 | 116 |
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