ssr_primer
Primer Details for TRINITY_DN13211_c0_g1_i3 |
|---|
| (TG)12: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCACCACTTACGCCTCAAGT | GGCGGCGGCTATAGAATTCT | Start: 1197 |
| Tm | 59.965 | 60.037 | End: 1312 |
| Size | 20 | 20 | 116 |
| Primer 2 details |
|---|
| Sequence | AAAAGTTGAATTCCGCCGCC | ACTTGAGGCGTAAGTGGTGG | Start: 1077 |
| Tm | 60.039 | 59.965 | End: 1216 |
| Size | 20 | 20 | 140 |
| Primer 3 details |
|---|
| Sequence | TTCCGACTGCTGATTCACCC | GGCGGCGGCTATAGAATTCT | Start: 1155 |
| Tm | 60.036 | 60.037 | End: 1312 |
| Size | 20 | 20 | 158 |
| Primer 4 details |
|---|
| Sequence | AAAAGTTGAATTCCGCCGCC | GGCGGCGGCTATAGAATTCT | Start: 1077 |
| Tm | 60.039 | 60.037 | End: 1312 |
| Size | 20 | 20 | 236 |
| Primer 5 details |
|---|
| Sequence | AGAATTCTATAGCCGCCGCC | CGCCTTCTTCAAACAGCCAC | Start: 1293 |
| Tm | 60.037 | 60.04 | End: 1565 |
| Size | 20 | 20 | 273 |
(GAA)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCACCACTTACGCCTCAAGT | GGCGGCGGCTATAGAATTCT | Start: 1197 |
| Tm | 59.965 | 60.037 | End: 1312 |
| Size | 20 | 20 | 116 |
| Primer 2 details |
|---|
| Sequence | AAAAGTTGAATTCCGCCGCC | ACTTGAGGCGTAAGTGGTGG | Start: 1077 |
| Tm | 60.039 | 59.965 | End: 1216 |
| Size | 20 | 20 | 140 |
| Primer 3 details |
|---|
| Sequence | TTCCGACTGCTGATTCACCC | GGCGGCGGCTATAGAATTCT | Start: 1155 |
| Tm | 60.036 | 60.037 | End: 1312 |
| Size | 20 | 20 | 158 |
| Primer 4 details |
|---|
| Sequence | AAAAGTTGAATTCCGCCGCC | GGCGGCGGCTATAGAATTCT | Start: 1077 |
| Tm | 60.039 | 60.037 | End: 1312 |
| Size | 20 | 20 | 236 |
| Primer 5 details |
|---|
| Sequence | AGAATTCTATAGCCGCCGCC | CGCCTTCTTCAAACAGCCAC | Start: 1293 |
| Tm | 60.037 | 60.04 | End: 1565 |
| Size | 20 | 20 | 273 |
(GCA)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCACCACTTACGCCTCAAGT | GGCGGCGGCTATAGAATTCT | Start: 1197 |
| Tm | 59.965 | 60.037 | End: 1312 |
| Size | 20 | 20 | 116 |
| Primer 2 details |
|---|
| Sequence | AAAAGTTGAATTCCGCCGCC | ACTTGAGGCGTAAGTGGTGG | Start: 1077 |
| Tm | 60.039 | 59.965 | End: 1216 |
| Size | 20 | 20 | 140 |
| Primer 3 details |
|---|
| Sequence | TTCCGACTGCTGATTCACCC | GGCGGCGGCTATAGAATTCT | Start: 1155 |
| Tm | 60.036 | 60.037 | End: 1312 |
| Size | 20 | 20 | 158 |
| Primer 4 details |
|---|
| Sequence | AAAAGTTGAATTCCGCCGCC | GGCGGCGGCTATAGAATTCT | Start: 1077 |
| Tm | 60.039 | 60.037 | End: 1312 |
| Size | 20 | 20 | 236 |
| Primer 5 details |
|---|
| Sequence | AGAATTCTATAGCCGCCGCC | CGCCTTCTTCAAACAGCCAC | Start: 1293 |
| Tm | 60.037 | 60.04 | End: 1565 |
| Size | 20 | 20 | 273 |
(A)14: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCACCACTTACGCCTCAAGT | GGCGGCGGCTATAGAATTCT | Start: 1197 |
| Tm | 59.965 | 60.037 | End: 1312 |
| Size | 20 | 20 | 116 |
| Primer 2 details |
|---|
| Sequence | AAAAGTTGAATTCCGCCGCC | ACTTGAGGCGTAAGTGGTGG | Start: 1077 |
| Tm | 60.039 | 59.965 | End: 1216 |
| Size | 20 | 20 | 140 |
| Primer 3 details |
|---|
| Sequence | TTCCGACTGCTGATTCACCC | GGCGGCGGCTATAGAATTCT | Start: 1155 |
| Tm | 60.036 | 60.037 | End: 1312 |
| Size | 20 | 20 | 158 |
| Primer 4 details |
|---|
| Sequence | AAAAGTTGAATTCCGCCGCC | GGCGGCGGCTATAGAATTCT | Start: 1077 |
| Tm | 60.039 | 60.037 | End: 1312 |
| Size | 20 | 20 | 236 |
| Primer 5 details |
|---|
| Sequence | AGAATTCTATAGCCGCCGCC | CGCCTTCTTCAAACAGCCAC | Start: 1293 |
| Tm | 60.037 | 60.04 | End: 1565 |
| Size | 20 | 20 | 273 |
(A)15: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCACCACTTACGCCTCAAGT | GGCGGCGGCTATAGAATTCT | Start: 1197 |
| Tm | 59.965 | 60.037 | End: 1312 |
| Size | 20 | 20 | 116 |
| Primer 2 details |
|---|
| Sequence | AAAAGTTGAATTCCGCCGCC | ACTTGAGGCGTAAGTGGTGG | Start: 1077 |
| Tm | 60.039 | 59.965 | End: 1216 |
| Size | 20 | 20 | 140 |
| Primer 3 details |
|---|
| Sequence | TTCCGACTGCTGATTCACCC | GGCGGCGGCTATAGAATTCT | Start: 1155 |
| Tm | 60.036 | 60.037 | End: 1312 |
| Size | 20 | 20 | 158 |
| Primer 4 details |
|---|
| Sequence | AAAAGTTGAATTCCGCCGCC | GGCGGCGGCTATAGAATTCT | Start: 1077 |
| Tm | 60.039 | 60.037 | End: 1312 |
| Size | 20 | 20 | 236 |
| Primer 5 details |
|---|
| Sequence | AGAATTCTATAGCCGCCGCC | CGCCTTCTTCAAACAGCCAC | Start: 1293 |
| Tm | 60.037 | 60.04 | End: 1565 |
| Size | 20 | 20 | 273 |
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