Primer Details for TRINITY_DN13208_c4_g5_i1 |
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| (A)22: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCCAAGCCAGTTCTTTGTTT | AGAGAAACTTGACAGAATAGGCAGT | Start: 47 |
| Tm | 57.398 | 59.987 | End: 226 |
| Size | 20 | 25 | 180 |
| Primer 2 details |
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| Sequence | GCCAGTTCTTTGTTTAAGCCCT | AGAGAAACTTGACAGAATAGGCAGT | Start: 52 |
| Tm | 59.372 | 59.987 | End: 226 |
| Size | 22 | 25 | 175 |
| Primer 3 details |
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| Sequence | AGCCAGTTCTTTGTTTAAGCCC | AGAGAAACTTGACAGAATAGGCAGT | Start: 51 |
| Tm | 59.372 | 59.987 | End: 226 |
| Size | 22 | 25 | 176 |
| Primer 4 details |
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| Sequence | GCCAAGCCAGTTCTTTGTTT | AGAAACTTGACAGAATAGGCAGT | Start: 47 |
| Tm | 57.398 | 57.766 | End: 224 |
| Size | 20 | 23 | 178 |
| Primer 5 details |
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| Sequence | GCCAAGCCAGTTCTTTGTTT | GAGAAACTTGACAGAATAGGCAGT | Start: 47 |
| Tm | 57.398 | 58.758 | End: 225 |
| Size | 20 | 24 | 179 |
