ssr_primer
Primer Details for TRINITY_DN13199_c2_g1_i5 |
|---|
| (AT)8: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGCTGCTTGCAGAGTAACGT | GTTTCCTCCCACGCCAGTTA | Start: 1956 |
| Tm | 59.966 | 59.964 | End: 2200 |
| Size | 20 | 20 | 245 |
| Primer 2 details |
|---|
| Sequence | CCTGGCAATTCCCATCACCT | ACTCCTCGGGTTAATGGGGA | Start: 3100 |
| Tm | 60.033 | 59.956 | End: 3226 |
| Size | 20 | 20 | 127 |
| Primer 3 details |
|---|
| Sequence | GGGATGCGGACGATGGTATT | TGGCTGAGCTTTTGGTGTGA | Start: 489 |
| Tm | 59.965 | 60.107 | End: 621 |
| Size | 20 | 20 | 133 |
| Primer 4 details |
|---|
| Sequence | AACCTCACACTCTGGCGATG | CTCTATTTGGGTGCCGCTCA | Start: 4584 |
| Tm | 60.037 | 60.108 | End: 4748 |
| Size | 20 | 20 | 165 |
| Primer 5 details |
|---|
| Sequence | TGGCATACACACGAACTGCT | GCCTCAGAAACCTTCCCACA | Start: 1267 |
| Tm | 59.965 | 59.89 | End: 1505 |
| Size | 20 | 20 | 239 |
(TAT)6: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGCTGCTTGCAGAGTAACGT | GTTTCCTCCCACGCCAGTTA | Start: 1956 |
| Tm | 59.966 | 59.964 | End: 2200 |
| Size | 20 | 20 | 245 |
| Primer 2 details |
|---|
| Sequence | CCTGGCAATTCCCATCACCT | ACTCCTCGGGTTAATGGGGA | Start: 3100 |
| Tm | 60.033 | 59.956 | End: 3226 |
| Size | 20 | 20 | 127 |
| Primer 3 details |
|---|
| Sequence | GGGATGCGGACGATGGTATT | TGGCTGAGCTTTTGGTGTGA | Start: 489 |
| Tm | 59.965 | 60.107 | End: 621 |
| Size | 20 | 20 | 133 |
| Primer 4 details |
|---|
| Sequence | AACCTCACACTCTGGCGATG | CTCTATTTGGGTGCCGCTCA | Start: 4584 |
| Tm | 60.037 | 60.108 | End: 4748 |
| Size | 20 | 20 | 165 |
| Primer 5 details |
|---|
| Sequence | TGGCATACACACGAACTGCT | GCCTCAGAAACCTTCCCACA | Start: 1267 |
| Tm | 59.965 | 59.89 | End: 1505 |
| Size | 20 | 20 | 239 |
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