ssr_primer
Primer Details for TRINITY_DN13188_c1_g1_i28 |
|---|
| (CT)9: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGTGTTTGTGTGGCACTCCT | ATGCACGCACACACAAAGTC | Start: 6758 |
| Tm | 60.035 | 59.971 | End: 7025 |
| Size | 20 | 20 | 268 |
| Primer 2 details |
|---|
| Sequence | CGTAGGCCCAGCTGGTTTAA | TCAATTCCCACCATTCCCGG | Start: 6307 |
| Tm | 60.036 | 60.034 | End: 6411 |
| Size | 20 | 20 | 105 |
| Primer 3 details |
|---|
| Sequence | GGGCTGGAGTGAAGCTGATT | TCCAGATTCCGCTTCCCAAC | Start: 1140 |
| Tm | 60.035 | 60.035 | End: 1395 |
| Size | 20 | 20 | 256 |
| Primer 4 details |
|---|
| Sequence | AGCTCTACTGGAGAAGCCCA | AACAACACAACCAGGCCTGA | Start: 3895 |
| Tm | 59.958 | 60.034 | End: 4056 |
| Size | 20 | 20 | 162 |
| Primer 5 details |
|---|
| Sequence | CGTAGGCCCAGCTGGTTTAA | TCCAAGCCTCAATTCCCACC | Start: 6307 |
| Tm | 60.036 | 59.96 | End: 6419 |
| Size | 20 | 20 | 113 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGTGTTTGTGTGGCACTCCT | ATGCACGCACACACAAAGTC | Start: 6758 |
| Tm | 60.035 | 59.971 | End: 7025 |
| Size | 20 | 20 | 268 |
| Primer 2 details |
|---|
| Sequence | CGTAGGCCCAGCTGGTTTAA | TCAATTCCCACCATTCCCGG | Start: 6307 |
| Tm | 60.036 | 60.034 | End: 6411 |
| Size | 20 | 20 | 105 |
| Primer 3 details |
|---|
| Sequence | GGGCTGGAGTGAAGCTGATT | TCCAGATTCCGCTTCCCAAC | Start: 1140 |
| Tm | 60.035 | 60.035 | End: 1395 |
| Size | 20 | 20 | 256 |
| Primer 4 details |
|---|
| Sequence | AGCTCTACTGGAGAAGCCCA | AACAACACAACCAGGCCTGA | Start: 3895 |
| Tm | 59.958 | 60.034 | End: 4056 |
| Size | 20 | 20 | 162 |
| Primer 5 details |
|---|
| Sequence | CGTAGGCCCAGCTGGTTTAA | TCCAAGCCTCAATTCCCACC | Start: 6307 |
| Tm | 60.036 | 59.96 | End: 6419 |
| Size | 20 | 20 | 113 |
(TC)7: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGTGTTTGTGTGGCACTCCT | ATGCACGCACACACAAAGTC | Start: 6758 |
| Tm | 60.035 | 59.971 | End: 7025 |
| Size | 20 | 20 | 268 |
| Primer 2 details |
|---|
| Sequence | CGTAGGCCCAGCTGGTTTAA | TCAATTCCCACCATTCCCGG | Start: 6307 |
| Tm | 60.036 | 60.034 | End: 6411 |
| Size | 20 | 20 | 105 |
| Primer 3 details |
|---|
| Sequence | GGGCTGGAGTGAAGCTGATT | TCCAGATTCCGCTTCCCAAC | Start: 1140 |
| Tm | 60.035 | 60.035 | End: 1395 |
| Size | 20 | 20 | 256 |
| Primer 4 details |
|---|
| Sequence | AGCTCTACTGGAGAAGCCCA | AACAACACAACCAGGCCTGA | Start: 3895 |
| Tm | 59.958 | 60.034 | End: 4056 |
| Size | 20 | 20 | 162 |
| Primer 5 details |
|---|
| Sequence | CGTAGGCCCAGCTGGTTTAA | TCCAAGCCTCAATTCCCACC | Start: 6307 |
| Tm | 60.036 | 59.96 | End: 6419 |
| Size | 20 | 20 | 113 |
(T)11acgagagggaggggattagg(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGTGTTTGTGTGGCACTCCT | ATGCACGCACACACAAAGTC | Start: 6758 |
| Tm | 60.035 | 59.971 | End: 7025 |
| Size | 20 | 20 | 268 |
| Primer 2 details |
|---|
| Sequence | CGTAGGCCCAGCTGGTTTAA | TCAATTCCCACCATTCCCGG | Start: 6307 |
| Tm | 60.036 | 60.034 | End: 6411 |
| Size | 20 | 20 | 105 |
| Primer 3 details |
|---|
| Sequence | GGGCTGGAGTGAAGCTGATT | TCCAGATTCCGCTTCCCAAC | Start: 1140 |
| Tm | 60.035 | 60.035 | End: 1395 |
| Size | 20 | 20 | 256 |
| Primer 4 details |
|---|
| Sequence | AGCTCTACTGGAGAAGCCCA | AACAACACAACCAGGCCTGA | Start: 3895 |
| Tm | 59.958 | 60.034 | End: 4056 |
| Size | 20 | 20 | 162 |
| Primer 5 details |
|---|
| Sequence | CGTAGGCCCAGCTGGTTTAA | TCCAAGCCTCAATTCCCACC | Start: 6307 |
| Tm | 60.036 | 59.96 | End: 6419 |
| Size | 20 | 20 | 113 |
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