Primer Details for TRINITY_DN13174_c7_g1_i1 | |||
|---|---|---|---|
| (A)10: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGAAAAAGAAAAGGAGAGTTCAGGT | TGGACTCTAGACTCTTCTCCCA | Start: 35 |
| Tm | 58.573 | 59.08 | End: 303 |
| Size | 25 | 22 | 269 |
| Primer 2 details | |||
| Sequence | GAAAAAGAAAAGGAGAGTTCAGGT | TGGACTCTAGACTCTTCTCCCA | Start: 36 |
| Tm | 57.299 | 59.08 | End: 303 |
| Size | 24 | 22 | 268 |
| Primer 3 details | |||
| Sequence | AGAAAAGGAGAGTTCAGGTAGAAA | TGGACTCTAGACTCTTCTCCCA | Start: 41 |
| Tm | 57.251 | 59.08 | End: 303 |
| Size | 24 | 22 | 263 |
| Primer 4 details | |||
| Sequence | AAGAAAAGGAGAGTTCAGGTAGAA | TGGACTCTAGACTCTTCTCCCA | Start: 40 |
| Tm | 57.251 | 59.08 | End: 303 |
| Size | 24 | 22 | 264 |
| Primer 5 details | |||
| Sequence | AAAGAAAAGGAGAGTTCAGGTAGA | TGGACTCTAGACTCTTCTCCCA | Start: 39 |
| Tm | 57.251 | 59.08 | End: 303 |
| Size | 24 | 22 | 265 |
