Primer Details for TRINITY_DN13174_c5_g3_i1 | |||
|---|---|---|---|
| (T)11: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCGAAAGAAACCTTGTCCGT | AGATACTTGAATCGGACAACAGTCA | Start: 59 |
| Tm | 57.392 | 60.047 | End: 159 |
| Size | 20 | 25 | 101 |
| Primer 2 details | |||
| Sequence | TCGAAAGAAACCTTGTCCGT | GATACTTGAATCGGACAACAGTCA | Start: 59 |
| Tm | 57.392 | 58.831 | End: 158 |
| Size | 20 | 24 | 100 |
| Primer 3 details | |||
| Sequence | TCGAAAGAAACCTTGTCCGTT | AGATACTTGAATCGGACAACAGTCA | Start: 59 |
| Tm | 58.091 | 60.047 | End: 159 |
| Size | 21 | 25 | 101 |
| Primer 4 details | |||
| Sequence | TCGAAAGAAACCTTGTCCGT | AGATACTTGAATCGGACAACAGT | Start: 59 |
| Tm | 57.392 | 57.593 | End: 159 |
| Size | 20 | 23 | 101 |
| Primer 5 details | |||
| Sequence | TCGAAAGAAACCTTGTCCGT | AGATACTTGAATCGGACAACAGTC | Start: 59 |
| Tm | 57.392 | 58.588 | End: 159 |
| Size | 20 | 24 | 101 |
