ssr_primer
Primer Details for TRINITY_DN13173_c0_g1_i1 |
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| (GAG)7: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AACACCCATCCGAACCCTTC | GTGAGATCGGCGAATTGTGC | Start: 953 |
| Tm | 59.962 | 59.973 | End: 1102 |
| Size | 20 | 20 | 150 |
| Primer 2 details |
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| Sequence | TGATGATCACAAGCCCCACC | GGTCTTGGTGAGAAAGGGGG | Start: 417 |
| Tm | 60.034 | 59.962 | End: 680 |
| Size | 20 | 20 | 264 |
| Primer 3 details |
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| Sequence | TTGGGAATTCCTCACCGCTC | GGTCTTGGTGAGAAAGGGGG | Start: 480 |
| Tm | 60.035 | 59.962 | End: 680 |
| Size | 20 | 20 | 201 |
| Primer 4 details |
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| Sequence | ACATCGGTTCCACTTCCACC | CGTCTGAACAAGCTCCGAGT | Start: 1030 |
| Tm | 59.964 | 60.039 | End: 1163 |
| Size | 20 | 20 | 134 |
| Primer 5 details |
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| Sequence | CCGCTCTTTCTCCGGTTTCT | GGTCTTGGTGAGAAAGGGGG | Start: 450 |
| Tm | 60.037 | 59.962 | End: 680 |
| Size | 20 | 20 | 231 |
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