ssr_primer
Primer Details for TRINITY_DN13171_c0_g6_i8 |
|---|
| (GAAA)5: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCCCCATGCTCTTCCCAAAT | GCAGCATTCTTCGGATGCAG | Start: 410 |
| Tm | 60.032 | 59.971 | End: 688 |
| Size | 20 | 20 | 279 |
| Primer 2 details |
|---|
| Sequence | CGACAGCGTTTCCTCACTCT | ATTTGGGAAGAGCATGGGGG | Start: 194 |
| Tm | 60.039 | 60.032 | End: 429 |
| Size | 20 | 20 | 236 |
| Primer 3 details |
|---|
| Sequence | GGCGGTGGTCTATCAGCTAC | AGAGTGAGGAAACGCTGTCG | Start: 110 |
| Tm | 59.968 | 60.039 | End: 213 |
| Size | 20 | 20 | 104 |
| Primer 4 details |
|---|
| Sequence | CCGCCCTAAACCACCATTCT | AGAGTGAGGAAACGCTGTCG | Start: 46 |
| Tm | 60.034 | 60.039 | End: 213 |
| Size | 20 | 20 | 168 |
| Primer 5 details |
|---|
| Sequence | AAATGGAAAATGCCACCGCC | AGAGTGAGGAAACGCTGTCG | Start: 31 |
| Tm | 60.036 | 60.039 | End: 213 |
| Size | 20 | 20 | 183 |
(AAG)9: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCCCCATGCTCTTCCCAAAT | GCAGCATTCTTCGGATGCAG | Start: 410 |
| Tm | 60.032 | 59.971 | End: 688 |
| Size | 20 | 20 | 279 |
| Primer 2 details |
|---|
| Sequence | CGACAGCGTTTCCTCACTCT | ATTTGGGAAGAGCATGGGGG | Start: 194 |
| Tm | 60.039 | 60.032 | End: 429 |
| Size | 20 | 20 | 236 |
| Primer 3 details |
|---|
| Sequence | GGCGGTGGTCTATCAGCTAC | AGAGTGAGGAAACGCTGTCG | Start: 110 |
| Tm | 59.968 | 60.039 | End: 213 |
| Size | 20 | 20 | 104 |
| Primer 4 details |
|---|
| Sequence | CCGCCCTAAACCACCATTCT | AGAGTGAGGAAACGCTGTCG | Start: 46 |
| Tm | 60.034 | 60.039 | End: 213 |
| Size | 20 | 20 | 168 |
| Primer 5 details |
|---|
| Sequence | AAATGGAAAATGCCACCGCC | AGAGTGAGGAAACGCTGTCG | Start: 31 |
| Tm | 60.036 | 60.039 | End: 213 |
| Size | 20 | 20 | 183 |
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