ssr_primer
Primer Details for TRINITY_DN13164_c0_g2_i2 |
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| (ATG)5: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGGAGTAATCGGAGCTGGA | CGCCAAGGGATCCAAGACTT | Start: 517 |
| Tm | 60.033 | 60.035 | End: 719 |
| Size | 20 | 20 | 203 |
| Primer 2 details |
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| Sequence | ACTACTCGTTGCTGGTTGGG | CTTTGCCCCACCAAGAATGC | Start: 6985 |
| Tm | 59.965 | 60.037 | End: 7121 |
| Size | 20 | 20 | 137 |
| Primer 3 details |
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| Sequence | TTTTCTGCAGAGGGGAGCTG | CGCCAAGGGATCCAAGACTT | Start: 493 |
| Tm | 59.963 | 60.035 | End: 719 |
| Size | 20 | 20 | 227 |
| Primer 4 details |
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| Sequence | ACTACTCGTTGCTGGTTGGG | CGGAAACCATTGGTCTGGGA | Start: 6985 |
| Tm | 59.965 | 59.962 | End: 7220 |
| Size | 20 | 20 | 236 |
| Primer 5 details |
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| Sequence | GTTTTCTGCAGAGGGGAGCT | CGCCAAGGGATCCAAGACTT | Start: 492 |
| Tm | 59.963 | 60.035 | End: 719 |
| Size | 20 | 20 | 228 |
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