ssr_primer
Primer Details for TRINITY_DN13160_c0_g1_i1 |
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| (T)18: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CACGTCGCCGGGATTTAAGA | ACGTGGAAACCTGCTCACAA | Start: 197 |
| Tm | 60.459 | 60.107 | End: 450 |
| Size | 20 | 20 | 254 |
| Primer 2 details |
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| Sequence | CACGTCGCCGGGATTTAAGA | AACGTGGAAACCTGCTCACA | Start: 197 |
| Tm | 60.459 | 60.107 | End: 451 |
| Size | 20 | 20 | 255 |
| Primer 3 details |
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| Sequence | ACGTCGCCGGGATTTAAGAT | ACGTGGAAACCTGCTCACAA | Start: 198 |
| Tm | 59.537 | 60.107 | End: 450 |
| Size | 20 | 20 | 253 |
| Primer 4 details |
|---|
| Sequence | ACGTCGCCGGGATTTAAGAT | AACGTGGAAACCTGCTCACA | Start: 198 |
| Tm | 59.537 | 60.107 | End: 451 |
| Size | 20 | 20 | 254 |
| Primer 5 details |
|---|
| Sequence | CACGTCGCCGGGATTTAAGA | CGTGGAAACCTGCTCACAAG | Start: 197 |
| Tm | 60.459 | 59.412 | End: 449 |
| Size | 20 | 20 | 253 |
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