ssr_primer
Primer Details for TRINITY_DN13158_c3_g4_i2 |
|---|
| (AAT)7: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGTCTCCCCCACTTCTCCTA | CGATACTTCCCGGCGATTCA | Start: 442 |
| Tm | 60.033 | 59.968 | End: 698 |
| Size | 20 | 20 | 257 |
| Primer 2 details |
|---|
| Sequence | AGAGTGGAGGGAGGATGCAT | GTGGCTTCCAAGGTCTCCTC | Start: 118 |
| Tm | 60.03 | 60.036 | End: 315 |
| Size | 20 | 20 | 198 |
| Primer 3 details |
|---|
| Sequence | ACATGGTCCGACGAGGTTTC | CGGCGATTAAGGTGCAATGG | Start: 1917 |
| Tm | 60.038 | 59.971 | End: 2042 |
| Size | 20 | 20 | 126 |
| Primer 4 details |
|---|
| Sequence | GACATGGTCCGACGAGGTTT | CGGCGATTAAGGTGCAATGG | Start: 1916 |
| Tm | 60.038 | 59.971 | End: 2042 |
| Size | 20 | 20 | 127 |
| Primer 5 details |
|---|
| Sequence | AGAGTGGAGGGAGGATGCAT | GAGGACACGGTTACCAGTGG | Start: 118 |
| Tm | 60.03 | 60.038 | End: 382 |
| Size | 20 | 20 | 265 |
(TC)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGTCTCCCCCACTTCTCCTA | CGATACTTCCCGGCGATTCA | Start: 442 |
| Tm | 60.033 | 59.968 | End: 698 |
| Size | 20 | 20 | 257 |
| Primer 2 details |
|---|
| Sequence | AGAGTGGAGGGAGGATGCAT | GTGGCTTCCAAGGTCTCCTC | Start: 118 |
| Tm | 60.03 | 60.036 | End: 315 |
| Size | 20 | 20 | 198 |
| Primer 3 details |
|---|
| Sequence | ACATGGTCCGACGAGGTTTC | CGGCGATTAAGGTGCAATGG | Start: 1917 |
| Tm | 60.038 | 59.971 | End: 2042 |
| Size | 20 | 20 | 126 |
| Primer 4 details |
|---|
| Sequence | GACATGGTCCGACGAGGTTT | CGGCGATTAAGGTGCAATGG | Start: 1916 |
| Tm | 60.038 | 59.971 | End: 2042 |
| Size | 20 | 20 | 127 |
| Primer 5 details |
|---|
| Sequence | AGAGTGGAGGGAGGATGCAT | GAGGACACGGTTACCAGTGG | Start: 118 |
| Tm | 60.03 | 60.038 | End: 382 |
| Size | 20 | 20 | 265 |
(TTCT)7: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGTCTCCCCCACTTCTCCTA | CGATACTTCCCGGCGATTCA | Start: 442 |
| Tm | 60.033 | 59.968 | End: 698 |
| Size | 20 | 20 | 257 |
| Primer 2 details |
|---|
| Sequence | AGAGTGGAGGGAGGATGCAT | GTGGCTTCCAAGGTCTCCTC | Start: 118 |
| Tm | 60.03 | 60.036 | End: 315 |
| Size | 20 | 20 | 198 |
| Primer 3 details |
|---|
| Sequence | ACATGGTCCGACGAGGTTTC | CGGCGATTAAGGTGCAATGG | Start: 1917 |
| Tm | 60.038 | 59.971 | End: 2042 |
| Size | 20 | 20 | 126 |
| Primer 4 details |
|---|
| Sequence | GACATGGTCCGACGAGGTTT | CGGCGATTAAGGTGCAATGG | Start: 1916 |
| Tm | 60.038 | 59.971 | End: 2042 |
| Size | 20 | 20 | 127 |
| Primer 5 details |
|---|
| Sequence | AGAGTGGAGGGAGGATGCAT | GAGGACACGGTTACCAGTGG | Start: 118 |
| Tm | 60.03 | 60.038 | End: 382 |
| Size | 20 | 20 | 265 |
(T)20: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGTCTCCCCCACTTCTCCTA | CGATACTTCCCGGCGATTCA | Start: 442 |
| Tm | 60.033 | 59.968 | End: 698 |
| Size | 20 | 20 | 257 |
| Primer 2 details |
|---|
| Sequence | AGAGTGGAGGGAGGATGCAT | GTGGCTTCCAAGGTCTCCTC | Start: 118 |
| Tm | 60.03 | 60.036 | End: 315 |
| Size | 20 | 20 | 198 |
| Primer 3 details |
|---|
| Sequence | ACATGGTCCGACGAGGTTTC | CGGCGATTAAGGTGCAATGG | Start: 1917 |
| Tm | 60.038 | 59.971 | End: 2042 |
| Size | 20 | 20 | 126 |
| Primer 4 details |
|---|
| Sequence | GACATGGTCCGACGAGGTTT | CGGCGATTAAGGTGCAATGG | Start: 1916 |
| Tm | 60.038 | 59.971 | End: 2042 |
| Size | 20 | 20 | 127 |
| Primer 5 details |
|---|
| Sequence | AGAGTGGAGGGAGGATGCAT | GAGGACACGGTTACCAGTGG | Start: 118 |
| Tm | 60.03 | 60.038 | End: 382 |
| Size | 20 | 20 | 265 |
(A)10gagattgtggggttgtgaatttgtgtggttttttttctttc(T)13: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGTCTCCCCCACTTCTCCTA | CGATACTTCCCGGCGATTCA | Start: 442 |
| Tm | 60.033 | 59.968 | End: 698 |
| Size | 20 | 20 | 257 |
| Primer 2 details |
|---|
| Sequence | AGAGTGGAGGGAGGATGCAT | GTGGCTTCCAAGGTCTCCTC | Start: 118 |
| Tm | 60.03 | 60.036 | End: 315 |
| Size | 20 | 20 | 198 |
| Primer 3 details |
|---|
| Sequence | ACATGGTCCGACGAGGTTTC | CGGCGATTAAGGTGCAATGG | Start: 1917 |
| Tm | 60.038 | 59.971 | End: 2042 |
| Size | 20 | 20 | 126 |
| Primer 4 details |
|---|
| Sequence | GACATGGTCCGACGAGGTTT | CGGCGATTAAGGTGCAATGG | Start: 1916 |
| Tm | 60.038 | 59.971 | End: 2042 |
| Size | 20 | 20 | 127 |
| Primer 5 details |
|---|
| Sequence | AGAGTGGAGGGAGGATGCAT | GAGGACACGGTTACCAGTGG | Start: 118 |
| Tm | 60.03 | 60.038 | End: 382 |
| Size | 20 | 20 | 265 |
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