ssr_primer
Primer Details for TRINITY_DN13133_c1_g1_i11 |
|---|
| (CAA)5: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACAAACCACCCACTGCAGAA | GATGTTGGAAGGTGGGTCGT | Start: 175 |
| Tm | 60.034 | 59.964 | End: 288 |
| Size | 20 | 20 | 114 |
| Primer 2 details |
|---|
| Sequence | AACAAACCACCCACTGCAGA | GATGTTGGAAGGTGGGTCGT | Start: 174 |
| Tm | 60.034 | 59.964 | End: 288 |
| Size | 20 | 20 | 115 |
| Primer 3 details |
|---|
| Sequence | CCCACTGCAGAAACTACCGT | GATGTTGGAAGGTGGGTCGT | Start: 183 |
| Tm | 59.965 | 59.964 | End: 288 |
| Size | 20 | 20 | 106 |
| Primer 4 details |
|---|
| Sequence | ACCCACTGCAGAAACTACCG | GATGTTGGAAGGTGGGTCGT | Start: 182 |
| Tm | 59.965 | 59.964 | End: 288 |
| Size | 20 | 20 | 107 |
| Primer 5 details |
|---|
| Sequence | ACGACCCACCTTCCAACATC | CGGGGTTGATTCCCTCCAAA | Start: 269 |
| Tm | 59.964 | 59.961 | End: 517 |
| Size | 20 | 20 | 249 |
(T)10ggttactgttaattttttaaaaagcaag(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACAAACCACCCACTGCAGAA | GATGTTGGAAGGTGGGTCGT | Start: 175 |
| Tm | 60.034 | 59.964 | End: 288 |
| Size | 20 | 20 | 114 |
| Primer 2 details |
|---|
| Sequence | AACAAACCACCCACTGCAGA | GATGTTGGAAGGTGGGTCGT | Start: 174 |
| Tm | 60.034 | 59.964 | End: 288 |
| Size | 20 | 20 | 115 |
| Primer 3 details |
|---|
| Sequence | CCCACTGCAGAAACTACCGT | GATGTTGGAAGGTGGGTCGT | Start: 183 |
| Tm | 59.965 | 59.964 | End: 288 |
| Size | 20 | 20 | 106 |
| Primer 4 details |
|---|
| Sequence | ACCCACTGCAGAAACTACCG | GATGTTGGAAGGTGGGTCGT | Start: 182 |
| Tm | 59.965 | 59.964 | End: 288 |
| Size | 20 | 20 | 107 |
| Primer 5 details |
|---|
| Sequence | ACGACCCACCTTCCAACATC | CGGGGTTGATTCCCTCCAAA | Start: 269 |
| Tm | 59.964 | 59.961 | End: 517 |
| Size | 20 | 20 | 249 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACAAACCACCCACTGCAGAA | GATGTTGGAAGGTGGGTCGT | Start: 175 |
| Tm | 60.034 | 59.964 | End: 288 |
| Size | 20 | 20 | 114 |
| Primer 2 details |
|---|
| Sequence | AACAAACCACCCACTGCAGA | GATGTTGGAAGGTGGGTCGT | Start: 174 |
| Tm | 60.034 | 59.964 | End: 288 |
| Size | 20 | 20 | 115 |
| Primer 3 details |
|---|
| Sequence | CCCACTGCAGAAACTACCGT | GATGTTGGAAGGTGGGTCGT | Start: 183 |
| Tm | 59.965 | 59.964 | End: 288 |
| Size | 20 | 20 | 106 |
| Primer 4 details |
|---|
| Sequence | ACCCACTGCAGAAACTACCG | GATGTTGGAAGGTGGGTCGT | Start: 182 |
| Tm | 59.965 | 59.964 | End: 288 |
| Size | 20 | 20 | 107 |
| Primer 5 details |
|---|
| Sequence | ACGACCCACCTTCCAACATC | CGGGGTTGATTCCCTCCAAA | Start: 269 |
| Tm | 59.964 | 59.961 | End: 517 |
| Size | 20 | 20 | 249 |
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