Primer Details for TRINITY_DN13129_c3_g1_i1 | |||
|---|---|---|---|
| (A)10: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCGATCAAGGTACTGGTTGC | AAAAGAATCATTTGGTTGCCTTTGG | Start: 84 |
| Tm | 59.273 | 59.468 | End: 183 |
| Size | 20 | 25 | 100 |
| Primer 2 details | |||
| Sequence | GTGGAAAAAGGAAAGAGCATAAGA | GGCAAACAAAATGGGGGCAT | Start: 0 |
| Tm | 57.425 | 59.962 | End: 133 |
| Size | 24 | 20 | 134 |
| Primer 3 details | |||
| Sequence | AAAGCGATCAAGGTACTGGTTG | AGAATCATTTGGTTGCCTTTGGT | Start: 81 |
| Tm | 58.926 | 59.352 | End: 180 |
| Size | 22 | 23 | 100 |
| Primer 4 details | |||
| Sequence | AGCGATCAAGGTACTGGTTGC | AAAGAATCATTTGGTTGCCTTTGG | Start: 83 |
| Tm | 60.676 | 58.935 | End: 182 |
| Size | 21 | 24 | 100 |
| Primer 5 details | |||
| Sequence | AAGCGATCAAGGTACTGGTTG | AAGAATCATTTGGTTGCCTTTGGT | Start: 82 |
| Tm | 58.3 | 59.897 | End: 181 |
| Size | 21 | 24 | 100 |
