ssr_primer
Primer Details for TRINITY_DN13121_c1_g1_i5 |
|---|
| (TC)7: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCCAATTCTCCCAGCGAAGT | AAGCTTAACGGCAGAGGAGG | Start: 8 |
| Tm | 60.035 | 59.749 | End: 133 |
| Size | 20 | 20 | 126 |
| Primer 2 details |
|---|
| Sequence | GCGAAGTCCCCATCTCCAAT | AAGCTTAACGGCAGAGGAGG | Start: 21 |
| Tm | 59.819 | 59.749 | End: 133 |
| Size | 20 | 20 | 113 |
| Primer 3 details |
|---|
| Sequence | AATTCTCCCAGCGAAGTCCC | AAGCTTAACGGCAGAGGAGG | Start: 11 |
| Tm | 59.747 | 59.749 | End: 133 |
| Size | 20 | 20 | 123 |
| Primer 4 details |
|---|
| Sequence | TTTCCCCAATTCTCCCAGCG | AAGCTTAACGGCAGAGGAGG | Start: 4 |
| Tm | 60.323 | 59.749 | End: 133 |
| Size | 20 | 20 | 130 |
| Primer 5 details |
|---|
| Sequence | CCCAATTCTCCCAGCGAAGT | TTAACGGCAGAGGAGGTGAC | Start: 8 |
| Tm | 60.035 | 59.393 | End: 129 |
| Size | 20 | 20 | 122 |
(TTTC)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCCAATTCTCCCAGCGAAGT | AAGCTTAACGGCAGAGGAGG | Start: 8 |
| Tm | 60.035 | 59.749 | End: 133 |
| Size | 20 | 20 | 126 |
| Primer 2 details |
|---|
| Sequence | GCGAAGTCCCCATCTCCAAT | AAGCTTAACGGCAGAGGAGG | Start: 21 |
| Tm | 59.819 | 59.749 | End: 133 |
| Size | 20 | 20 | 113 |
| Primer 3 details |
|---|
| Sequence | AATTCTCCCAGCGAAGTCCC | AAGCTTAACGGCAGAGGAGG | Start: 11 |
| Tm | 59.747 | 59.749 | End: 133 |
| Size | 20 | 20 | 123 |
| Primer 4 details |
|---|
| Sequence | TTTCCCCAATTCTCCCAGCG | AAGCTTAACGGCAGAGGAGG | Start: 4 |
| Tm | 60.323 | 59.749 | End: 133 |
| Size | 20 | 20 | 130 |
| Primer 5 details |
|---|
| Sequence | CCCAATTCTCCCAGCGAAGT | TTAACGGCAGAGGAGGTGAC | Start: 8 |
| Tm | 60.035 | 59.393 | End: 129 |
| Size | 20 | 20 | 122 |
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