ssr_primer
Primer Details for TRINITY_DN13113_c2_g1_i2 |
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| (A)11: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCCTCTTGTTCTGCAAACGG | GTGAAGAAGCGGACAGACGA | Start: 3407 |
| Tm | 60.04 | 60.039 | End: 3573 |
| Size | 20 | 20 | 167 |
| Primer 2 details |
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| Sequence | CTTCAGAACTGGATGCCCGT | CCACGCAAGCAATCATCTGG | Start: 3921 |
| Tm | 60.036 | 59.9 | End: 4200 |
| Size | 20 | 20 | 280 |
| Primer 3 details |
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| Sequence | CCTGCTCTCTGATTTGCCGA | CCCGATGGAGCAAGGAAAGT | Start: 1532 |
| Tm | 60.108 | 60.035 | End: 1713 |
| Size | 20 | 20 | 182 |
| Primer 4 details |
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| Sequence | CCTGCTCTCTGATTTGCCGA | ACCCGATGGAGCAAGGAAAG | Start: 1532 |
| Tm | 60.108 | 60.035 | End: 1714 |
| Size | 20 | 20 | 183 |
| Primer 5 details |
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| Sequence | CCAGAACGACCTGCTCTCTG | CCCGATGGAGCAAGGAAAGT | Start: 1523 |
| Tm | 60.109 | 60.035 | End: 1713 |
| Size | 20 | 20 | 191 |
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