Primer Details for TRINITY_DN13107_c3_g1_i1 | |||
|---|---|---|---|
| (T)12: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCGGTTTGAAAAAGATTTTGGGTCA | AGCCCTTCCACCAAATATCTGA | Start: 163 |
| Tm | 60.105 | 59.148 | End: 271 |
| Size | 25 | 22 | 109 |
| Primer 2 details | |||
| Sequence | CGGTTTGAAAAAGATTTTGGGTCA | AGCCCTTCCACCAAATATCTGA | Start: 164 |
| Tm | 58.887 | 59.148 | End: 271 |
| Size | 24 | 22 | 108 |
| Primer 3 details | |||
| Sequence | TCTTGCTTGGTTTAACAAAGACTGT | ACACACCTTGAAGGAAGATCCA | Start: 41 |
| Tm | 59.582 | 59.289 | End: 235 |
| Size | 25 | 22 | 195 |
| Primer 4 details | |||
| Sequence | TCGGTTTGAAAAAGATTTTGGGT | AGCCCTTCCACCAAATATCTGA | Start: 163 |
| Tm | 57.654 | 59.148 | End: 271 |
| Size | 23 | 22 | 109 |
| Primer 5 details | |||
| Sequence | TCGGTTTGAAAAAGATTTTGGGTC | AGCCCTTCCACCAAATATCTGA | Start: 163 |
| Tm | 58.647 | 59.148 | End: 271 |
| Size | 24 | 22 | 109 |
