Primer Details for TRINITY_DN13093_c2_g1_i1 |
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| (A)11: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACACACAATAATCAGAGTTTAGGAAC | CACTGGTGAGTTAAATCTTCAATCT | Start: 104 |
| Tm | 57.676 | 57.321 | End: 235 |
| Size | 26 | 25 | 132 |
| Primer 2 details |
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| Sequence | ACACACAATAATCAGAGTTTAGGAAC | CACTGGTGAGTTAAATCTTCAATCTA | Start: 104 |
| Tm | 57.676 | 57.222 | End: 235 |
| Size | 26 | 26 | 132 |
| Primer 3 details |
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| Sequence | TACACACAATAATCAGAGTTTAGGAAC | CACTGGTGAGTTAAATCTTCAATCT | Start: 103 |
| Tm | 57.568 | 57.321 | End: 235 |
| Size | 27 | 25 | 133 |
| Primer 4 details |
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| Sequence | CACACAATAATCAGAGTTTAGGAACTA | CACTGGTGAGTTAAATCTTCAATCT | Start: 105 |
| Tm | 57.347 | 57.321 | End: 235 |
| Size | 27 | 25 | 131 |
| Primer 5 details |
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| Sequence | ACACACAATAATCAGAGTTTAGGAAC | CACTGGTGAGTTAAATCTTCAATCTAT | Start: 104 |
| Tm | 57.676 | 57.611 | End: 235 |
| Size | 26 | 27 | 132 |
