ssr_primer
Primer Details for TRINITY_DN13088_c0_g1_i19 |
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| (CAT)6ccttcattcggccctctacgctataacgcagtttacccttccatctccaaacacccatattactgtatttcctctatt(A)16: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CAATTCGTCCTTGGGAGCCT | ATTTGCAGTGGGATCCTGGG | Start: 4225 |
| Tm | 60.035 | 60.033 | End: 4402 |
| Size | 20 | 20 | 178 |
| Primer 2 details |
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| Sequence | ATTTGCAGTGCAGCCAATGG | GCGGGTTGAGTTGTTTTCCC | Start: 2145 |
| Tm | 60.037 | 59.968 | End: 2393 |
| Size | 20 | 20 | 249 |
| Primer 3 details |
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| Sequence | AGTGCAGCCAATGGATGACA | GCGGGTTGAGTTGTTTTCCC | Start: 2151 |
| Tm | 59.961 | 59.968 | End: 2393 |
| Size | 20 | 20 | 243 |
| Primer 4 details |
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| Sequence | AACAGGTACCCTCGTCTCGA | CGTCTTGCTTGCATTGGCTT | Start: 1378 |
| Tm | 59.963 | 60.039 | End: 1627 |
| Size | 20 | 20 | 250 |
| Primer 5 details |
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| Sequence | AGTGCAGCCAATGGATGACA | CAGAGCTAGACACCGCACAA | Start: 2151 |
| Tm | 59.961 | 60.038 | End: 2428 |
| Size | 20 | 20 | 278 |
(TCT)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CAATTCGTCCTTGGGAGCCT | ATTTGCAGTGGGATCCTGGG | Start: 4225 |
| Tm | 60.035 | 60.033 | End: 4402 |
| Size | 20 | 20 | 178 |
| Primer 2 details |
|---|
| Sequence | ATTTGCAGTGCAGCCAATGG | GCGGGTTGAGTTGTTTTCCC | Start: 2145 |
| Tm | 60.037 | 59.968 | End: 2393 |
| Size | 20 | 20 | 249 |
| Primer 3 details |
|---|
| Sequence | AGTGCAGCCAATGGATGACA | GCGGGTTGAGTTGTTTTCCC | Start: 2151 |
| Tm | 59.961 | 59.968 | End: 2393 |
| Size | 20 | 20 | 243 |
| Primer 4 details |
|---|
| Sequence | AACAGGTACCCTCGTCTCGA | CGTCTTGCTTGCATTGGCTT | Start: 1378 |
| Tm | 59.963 | 60.039 | End: 1627 |
| Size | 20 | 20 | 250 |
| Primer 5 details |
|---|
| Sequence | AGTGCAGCCAATGGATGACA | CAGAGCTAGACACCGCACAA | Start: 2151 |
| Tm | 59.961 | 60.038 | End: 2428 |
| Size | 20 | 20 | 278 |
(T)16acaaaaagcaaaaatttcatcggtatatggaaaggaagaaaaaaatcaaagataaactcgcctc(GA)6: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CAATTCGTCCTTGGGAGCCT | ATTTGCAGTGGGATCCTGGG | Start: 4225 |
| Tm | 60.035 | 60.033 | End: 4402 |
| Size | 20 | 20 | 178 |
| Primer 2 details |
|---|
| Sequence | ATTTGCAGTGCAGCCAATGG | GCGGGTTGAGTTGTTTTCCC | Start: 2145 |
| Tm | 60.037 | 59.968 | End: 2393 |
| Size | 20 | 20 | 249 |
| Primer 3 details |
|---|
| Sequence | AGTGCAGCCAATGGATGACA | GCGGGTTGAGTTGTTTTCCC | Start: 2151 |
| Tm | 59.961 | 59.968 | End: 2393 |
| Size | 20 | 20 | 243 |
| Primer 4 details |
|---|
| Sequence | AACAGGTACCCTCGTCTCGA | CGTCTTGCTTGCATTGGCTT | Start: 1378 |
| Tm | 59.963 | 60.039 | End: 1627 |
| Size | 20 | 20 | 250 |
| Primer 5 details |
|---|
| Sequence | AGTGCAGCCAATGGATGACA | CAGAGCTAGACACCGCACAA | Start: 2151 |
| Tm | 59.961 | 60.038 | End: 2428 |
| Size | 20 | 20 | 278 |
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