ssr_primer
Primer Details for TRINITY_DN13082_c2_g2_i5 |
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| (T)10gcttccgactccttttcgcacattttgtcaagctttgtttttc(T)11: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCAACGCCCATCATGTTGA | ACGAGGAGAATGGGTGGAGA | Start: 276 |
| Tm | 59.963 | 59.959 | End: 385 |
| Size | 20 | 20 | 110 |
| Primer 2 details |
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| Sequence | TCCAACGCCCATCATGTTGA | ACTCCCAGGAGCTTCAGGAT | Start: 276 |
| Tm | 59.963 | 59.956 | End: 484 |
| Size | 20 | 20 | 209 |
| Primer 3 details |
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| Sequence | TCTCCACCCATTCTCCTCGT | ACTCCCAGGAGCTTCAGGAT | Start: 366 |
| Tm | 59.959 | 59.956 | End: 484 |
| Size | 20 | 20 | 119 |
| Primer 4 details |
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| Sequence | ACTCATGCGTGGTCACAACT | ATCCTTGGCCGCTCGAATAG | Start: 962 |
| Tm | 59.893 | 59.966 | End: 1193 |
| Size | 20 | 20 | 232 |
| Primer 5 details |
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| Sequence | TCTCCACCCATTCTCCTCGT | GACGTGCCGGAGCTTATACA | Start: 366 |
| Tm | 59.959 | 59.898 | End: 591 |
| Size | 20 | 20 | 226 |
(T)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCAACGCCCATCATGTTGA | ACGAGGAGAATGGGTGGAGA | Start: 276 |
| Tm | 59.963 | 59.959 | End: 385 |
| Size | 20 | 20 | 110 |
| Primer 2 details |
|---|
| Sequence | TCCAACGCCCATCATGTTGA | ACTCCCAGGAGCTTCAGGAT | Start: 276 |
| Tm | 59.963 | 59.956 | End: 484 |
| Size | 20 | 20 | 209 |
| Primer 3 details |
|---|
| Sequence | TCTCCACCCATTCTCCTCGT | ACTCCCAGGAGCTTCAGGAT | Start: 366 |
| Tm | 59.959 | 59.956 | End: 484 |
| Size | 20 | 20 | 119 |
| Primer 4 details |
|---|
| Sequence | ACTCATGCGTGGTCACAACT | ATCCTTGGCCGCTCGAATAG | Start: 962 |
| Tm | 59.893 | 59.966 | End: 1193 |
| Size | 20 | 20 | 232 |
| Primer 5 details |
|---|
| Sequence | TCTCCACCCATTCTCCTCGT | GACGTGCCGGAGCTTATACA | Start: 366 |
| Tm | 59.959 | 59.898 | End: 591 |
| Size | 20 | 20 | 226 |
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