ssr_primer
Primer Details for TRINITY_DN13053_c3_g2_i1 |
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| (AAC)6: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACCAGTGAATTAGGGGGATCA | GTTCCATGGGAAGTGGAGGG | Start: 196 |
| Tm | 58.141 | 60.034 | End: 351 |
| Size | 21 | 20 | 156 |
| Primer 2 details |
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| Sequence | ACCAGTGAATTAGGGGGATCA | GGTTCCATGGGAAGTGGAGG | Start: 196 |
| Tm | 58.141 | 60.034 | End: 352 |
| Size | 21 | 20 | 157 |
| Primer 3 details |
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| Sequence | AGGATACCAGTGAATTAGGGGGA | GTTCCATGGGAAGTGGAGGG | Start: 191 |
| Tm | 60.051 | 60.034 | End: 351 |
| Size | 23 | 20 | 161 |
| Primer 4 details |
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| Sequence | AGGATACCAGTGAATTAGGGGGA | GGTTCCATGGGAAGTGGAGG | Start: 191 |
| Tm | 60.051 | 60.034 | End: 352 |
| Size | 23 | 20 | 162 |
| Primer 5 details |
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| Sequence | GGCATCCCTGGCTAAACTTCT | CCTAGCCAACATGAGCATAGC | Start: 5 |
| Tm | 60.064 | 58.85 | End: 281 |
| Size | 21 | 21 | 277 |
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