ssr_primer
Primer Details for TRINITY_DN13049_c1_g3_i1 |
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| (TTA)8: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCCTCTCCCCTACCCAATGA | ACGAATGGGTAATGCAGGCA | Start: 728 |
| Tm | 60.03 | 60.035 | End: 871 |
| Size | 20 | 20 | 144 |
| Primer 2 details |
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| Sequence | AAACAACCCACAGAGAGCCC | ACGAATGGGTAATGCAGGCA | Start: 711 |
| Tm | 60.179 | 60.035 | End: 871 |
| Size | 20 | 20 | 161 |
| Primer 3 details |
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| Sequence | ACAAGAACCCCCAAACCACA | TCATTGGGTAGGGGAGAGGG | Start: 637 |
| Tm | 59.663 | 60.03 | End: 747 |
| Size | 20 | 20 | 111 |
| Primer 4 details |
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| Sequence | ACAAGAACCCCCAAACCACA | ACGAATGGGTAATGCAGGCA | Start: 637 |
| Tm | 59.663 | 60.035 | End: 871 |
| Size | 20 | 20 | 235 |
| Primer 5 details |
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| Sequence | GCAAGCCAAGGAAAACGACC | GGGTTGTTGGTTTGGCCTTG | Start: 134 |
| Tm | 60.319 | 60.179 | End: 331 |
| Size | 20 | 20 | 198 |
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