ssr_primer
Primer Details for TRINITY_DN13049_c0_g1_i1 |
|---|
| (T)13: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGGCCAGATTGCTGAATTGG | TGGGGGACTCAAAGAGGGAA | Start: 385 |
| Tm | 59.898 | 60.104 | End: 542 |
| Size | 20 | 20 | 158 |
| Primer 2 details |
|---|
| Sequence | ATGGTCACTAGGCTGGGACT | CGAAGCGACACTCTCTCGAA | Start: 2179 |
| Tm | 59.957 | 59.834 | End: 2436 |
| Size | 20 | 20 | 258 |
| Primer 3 details |
|---|
| Sequence | CGGCCAGATTGCTGAATTGG | AGGCCATGGATCGATCAACC | Start: 385 |
| Tm | 59.898 | 59.891 | End: 584 |
| Size | 20 | 20 | 200 |
| Primer 4 details |
|---|
| Sequence | CCTACGGCCAGATTGCTGAA | TGGGGGACTCAAAGAGGGAA | Start: 381 |
| Tm | 60.108 | 60.104 | End: 542 |
| Size | 20 | 20 | 162 |
| Primer 5 details |
|---|
| Sequence | CCTACGGCCAGATTGCTGAA | AGGCCATGGATCGATCAACC | Start: 381 |
| Tm | 60.108 | 59.891 | End: 584 |
| Size | 20 | 20 | 204 |
(T)12cttctctctatatgaagtatattactacttctggctgtgtttggttttagctactcacataaagaagtg(T)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGGCCAGATTGCTGAATTGG | TGGGGGACTCAAAGAGGGAA | Start: 385 |
| Tm | 59.898 | 60.104 | End: 542 |
| Size | 20 | 20 | 158 |
| Primer 2 details |
|---|
| Sequence | ATGGTCACTAGGCTGGGACT | CGAAGCGACACTCTCTCGAA | Start: 2179 |
| Tm | 59.957 | 59.834 | End: 2436 |
| Size | 20 | 20 | 258 |
| Primer 3 details |
|---|
| Sequence | CGGCCAGATTGCTGAATTGG | AGGCCATGGATCGATCAACC | Start: 385 |
| Tm | 59.898 | 59.891 | End: 584 |
| Size | 20 | 20 | 200 |
| Primer 4 details |
|---|
| Sequence | CCTACGGCCAGATTGCTGAA | TGGGGGACTCAAAGAGGGAA | Start: 381 |
| Tm | 60.108 | 60.104 | End: 542 |
| Size | 20 | 20 | 162 |
| Primer 5 details |
|---|
| Sequence | CCTACGGCCAGATTGCTGAA | AGGCCATGGATCGATCAACC | Start: 381 |
| Tm | 60.108 | 59.891 | End: 584 |
| Size | 20 | 20 | 204 |
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