Primer Details for TRINITY_DN13044_c8_g1_i1 |
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| (A)21: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCAACAAATTTTCAAACCTAGGGAAGT | TGTGATGTGTGAAGATGACTTAATT | Start: 87 |
| Tm | 59.875 | 57.016 | End: 201 |
| Size | 27 | 25 | 115 |
| Primer 2 details |
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| Sequence | TCAACAAATTTTCAAACCTAGGGAAGT | TTGTGATGTGTGAAGATGACTTAAT | Start: 87 |
| Tm | 59.875 | 57.016 | End: 202 |
| Size | 27 | 25 | 116 |
| Primer 3 details |
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| Sequence | CAACAAATTTTCAAACCTAGGGAAGT | TGTGATGTGTGAAGATGACTTAATT | Start: 88 |
| Tm | 58.728 | 57.016 | End: 201 |
| Size | 26 | 25 | 114 |
| Primer 4 details |
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| Sequence | CAACAAATTTTCAAACCTAGGGAAGT | TTGTGATGTGTGAAGATGACTTAAT | Start: 88 |
| Tm | 58.728 | 57.016 | End: 202 |
| Size | 26 | 25 | 115 |
| Primer 5 details |
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| Sequence | TCATCAACAAATTTTCAAACCTAGGGA | TGTGATGTGTGAAGATGACTTAATT | Start: 84 |
| Tm | 59.711 | 57.016 | End: 201 |
| Size | 27 | 25 | 118 |
