ssr_primer
Primer Details for TRINITY_DN13026_c5_g3_i1 |
|---|
| (T)12: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTGGGCATCTTCCAGGACAC | CTCAGCTCTCCTTGTGCCAA | Start: 234 |
| Tm | 59.962 | 59.964 | End: 398 |
| Size | 20 | 20 | 165 |
| Primer 2 details |
|---|
| Sequence | ATGGCCGTCCTGCTAATGAG | CTCAGCTCTCCTTGTGCCAA | Start: 174 |
| Tm | 59.893 | 59.964 | End: 398 |
| Size | 20 | 20 | 225 |
| Primer 3 details |
|---|
| Sequence | GGGTATGTGGCGCCAGAATA | GTGTCCTGGAAGATGCCCAA | Start: 62 |
| Tm | 59.892 | 59.962 | End: 253 |
| Size | 20 | 20 | 192 |
| Primer 4 details |
|---|
| Sequence | GTTGACACCCCATAGCGACA | CGAAACGCCTCATTGTGGTG | Start: 1911 |
| Tm | 60.037 | 60.11 | End: 2185 |
| Size | 20 | 20 | 275 |
| Primer 5 details |
|---|
| Sequence | ACCGGTACCTTTTTGGGTGG | TGCCCCTCCCAATCCATCTA | Start: 1709 |
| Tm | 60.179 | 60.029 | End: 1892 |
| Size | 20 | 20 | 184 |
(T)26: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTGGGCATCTTCCAGGACAC | CTCAGCTCTCCTTGTGCCAA | Start: 234 |
| Tm | 59.962 | 59.964 | End: 398 |
| Size | 20 | 20 | 165 |
| Primer 2 details |
|---|
| Sequence | ATGGCCGTCCTGCTAATGAG | CTCAGCTCTCCTTGTGCCAA | Start: 174 |
| Tm | 59.893 | 59.964 | End: 398 |
| Size | 20 | 20 | 225 |
| Primer 3 details |
|---|
| Sequence | GGGTATGTGGCGCCAGAATA | GTGTCCTGGAAGATGCCCAA | Start: 62 |
| Tm | 59.892 | 59.962 | End: 253 |
| Size | 20 | 20 | 192 |
| Primer 4 details |
|---|
| Sequence | GTTGACACCCCATAGCGACA | CGAAACGCCTCATTGTGGTG | Start: 1911 |
| Tm | 60.037 | 60.11 | End: 2185 |
| Size | 20 | 20 | 275 |
| Primer 5 details |
|---|
| Sequence | ACCGGTACCTTTTTGGGTGG | TGCCCCTCCCAATCCATCTA | Start: 1709 |
| Tm | 60.179 | 60.029 | End: 1892 |
| Size | 20 | 20 | 184 |
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