ssr_primer
Primer Details for TRINITY_DN13009_c0_g2_i21 |
|---|
| (AG)11(A)21: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCTTCCGCTTCTTCATCGT | ATGCAACTGCCAGAGAGCAT | Start: 324 |
| Tm | 60.037 | 60.034 | End: 534 |
| Size | 20 | 20 | 211 |
| Primer 2 details |
|---|
| Sequence | TGCTTCCGCTTCTTCATCGT | CCAGCAGTCAAATGTCCCCT | Start: 324 |
| Tm | 60.037 | 59.961 | End: 575 |
| Size | 20 | 20 | 252 |
| Primer 3 details |
|---|
| Sequence | ATTGGACCCTCTCTCCCTCC | GCTGGGAATGGCTATGACGA | Start: 1535 |
| Tm | 60.031 | 59.893 | End: 1813 |
| Size | 20 | 20 | 279 |
| Primer 4 details |
|---|
| Sequence | ATTGGACCCTCTCTCCCTCC | AGCTGGGAATGGCTATGACG | Start: 1535 |
| Tm | 60.031 | 59.893 | End: 1814 |
| Size | 20 | 20 | 280 |
| Primer 5 details |
|---|
| Sequence | TCGTCATAGCCATTCCCAGC | GAAAATGTGGGGGAGGGAGG | Start: 1794 |
| Tm | 59.893 | 60.033 | End: 2063 |
| Size | 20 | 20 | 270 |
(TCT)7(TCC)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCTTCCGCTTCTTCATCGT | ATGCAACTGCCAGAGAGCAT | Start: 324 |
| Tm | 60.037 | 60.034 | End: 534 |
| Size | 20 | 20 | 211 |
| Primer 2 details |
|---|
| Sequence | TGCTTCCGCTTCTTCATCGT | CCAGCAGTCAAATGTCCCCT | Start: 324 |
| Tm | 60.037 | 59.961 | End: 575 |
| Size | 20 | 20 | 252 |
| Primer 3 details |
|---|
| Sequence | ATTGGACCCTCTCTCCCTCC | GCTGGGAATGGCTATGACGA | Start: 1535 |
| Tm | 60.031 | 59.893 | End: 1813 |
| Size | 20 | 20 | 279 |
| Primer 4 details |
|---|
| Sequence | ATTGGACCCTCTCTCCCTCC | AGCTGGGAATGGCTATGACG | Start: 1535 |
| Tm | 60.031 | 59.893 | End: 1814 |
| Size | 20 | 20 | 280 |
| Primer 5 details |
|---|
| Sequence | TCGTCATAGCCATTCCCAGC | GAAAATGTGGGGGAGGGAGG | Start: 1794 |
| Tm | 59.893 | 60.033 | End: 2063 |
| Size | 20 | 20 | 270 |
(T)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCTTCCGCTTCTTCATCGT | ATGCAACTGCCAGAGAGCAT | Start: 324 |
| Tm | 60.037 | 60.034 | End: 534 |
| Size | 20 | 20 | 211 |
| Primer 2 details |
|---|
| Sequence | TGCTTCCGCTTCTTCATCGT | CCAGCAGTCAAATGTCCCCT | Start: 324 |
| Tm | 60.037 | 59.961 | End: 575 |
| Size | 20 | 20 | 252 |
| Primer 3 details |
|---|
| Sequence | ATTGGACCCTCTCTCCCTCC | GCTGGGAATGGCTATGACGA | Start: 1535 |
| Tm | 60.031 | 59.893 | End: 1813 |
| Size | 20 | 20 | 279 |
| Primer 4 details |
|---|
| Sequence | ATTGGACCCTCTCTCCCTCC | AGCTGGGAATGGCTATGACG | Start: 1535 |
| Tm | 60.031 | 59.893 | End: 1814 |
| Size | 20 | 20 | 280 |
| Primer 5 details |
|---|
| Sequence | TCGTCATAGCCATTCCCAGC | GAAAATGTGGGGGAGGGAGG | Start: 1794 |
| Tm | 59.893 | 60.033 | End: 2063 |
| Size | 20 | 20 | 270 |
(TAT)7: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCTTCCGCTTCTTCATCGT | ATGCAACTGCCAGAGAGCAT | Start: 324 |
| Tm | 60.037 | 60.034 | End: 534 |
| Size | 20 | 20 | 211 |
| Primer 2 details |
|---|
| Sequence | TGCTTCCGCTTCTTCATCGT | CCAGCAGTCAAATGTCCCCT | Start: 324 |
| Tm | 60.037 | 59.961 | End: 575 |
| Size | 20 | 20 | 252 |
| Primer 3 details |
|---|
| Sequence | ATTGGACCCTCTCTCCCTCC | GCTGGGAATGGCTATGACGA | Start: 1535 |
| Tm | 60.031 | 59.893 | End: 1813 |
| Size | 20 | 20 | 279 |
| Primer 4 details |
|---|
| Sequence | ATTGGACCCTCTCTCCCTCC | AGCTGGGAATGGCTATGACG | Start: 1535 |
| Tm | 60.031 | 59.893 | End: 1814 |
| Size | 20 | 20 | 280 |
| Primer 5 details |
|---|
| Sequence | TCGTCATAGCCATTCCCAGC | GAAAATGTGGGGGAGGGAGG | Start: 1794 |
| Tm | 59.893 | 60.033 | End: 2063 |
| Size | 20 | 20 | 270 |
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