ssr_primer
Primer Details for TRINITY_DN13008_c2_g1_i2 |
|---|
| (T)13: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTGATCTTCCCGTCCCTTGC | CCATCCTCCAGACTCCTCCA | Start: 1527 |
| Tm | 60.035 | 60.032 | End: 1767 |
| Size | 20 | 20 | 241 |
| Primer 2 details |
|---|
| Sequence | GGCCAGATGGTCTTTCGTGA | CCATCCTCCAGACTCCTCCA | Start: 1590 |
| Tm | 60.036 | 60.032 | End: 1767 |
| Size | 20 | 20 | 178 |
| Primer 3 details |
|---|
| Sequence | TTGGTTCAGGCCAGATGGTC | CCATCCTCCAGACTCCTCCA | Start: 1582 |
| Tm | 59.962 | 60.032 | End: 1767 |
| Size | 20 | 20 | 186 |
| Primer 4 details |
|---|
| Sequence | AGAACACCATGTCCGTCACC | TCAGCAGCAGCCAGTAAGTC | Start: 2201 |
| Tm | 59.965 | 60.037 | End: 2330 |
| Size | 20 | 20 | 130 |
| Primer 5 details |
|---|
| Sequence | GACTTACTGGCTGCTGCTGA | CTCCTTGTGCAGCCAAGAGA | Start: 2311 |
| Tm | 60.037 | 59.964 | End: 2533 |
| Size | 20 | 20 | 223 |
(T)20: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTGATCTTCCCGTCCCTTGC | CCATCCTCCAGACTCCTCCA | Start: 1527 |
| Tm | 60.035 | 60.032 | End: 1767 |
| Size | 20 | 20 | 241 |
| Primer 2 details |
|---|
| Sequence | GGCCAGATGGTCTTTCGTGA | CCATCCTCCAGACTCCTCCA | Start: 1590 |
| Tm | 60.036 | 60.032 | End: 1767 |
| Size | 20 | 20 | 178 |
| Primer 3 details |
|---|
| Sequence | TTGGTTCAGGCCAGATGGTC | CCATCCTCCAGACTCCTCCA | Start: 1582 |
| Tm | 59.962 | 60.032 | End: 1767 |
| Size | 20 | 20 | 186 |
| Primer 4 details |
|---|
| Sequence | AGAACACCATGTCCGTCACC | TCAGCAGCAGCCAGTAAGTC | Start: 2201 |
| Tm | 59.965 | 60.037 | End: 2330 |
| Size | 20 | 20 | 130 |
| Primer 5 details |
|---|
| Sequence | GACTTACTGGCTGCTGCTGA | CTCCTTGTGCAGCCAAGAGA | Start: 2311 |
| Tm | 60.037 | 59.964 | End: 2533 |
| Size | 20 | 20 | 223 |
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