ssr_primer
Primer Details for TRINITY_DN13001_c1_g1_i6 |
|---|
| (CTT)5: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTGGTCTGCTTGTGTCTGGA | GGTGCACTACAAAGCAACGG | Start: 905 |
| Tm | 59.893 | 60.04 | End: 1015 |
| Size | 20 | 20 | 111 |
| Primer 2 details |
|---|
| Sequence | GGGCCTGCACTCTACAAACT | CATCGACACGAGGCAACCTA | Start: 3311 |
| Tm | 59.963 | 59.827 | End: 3497 |
| Size | 20 | 20 | 187 |
| Primer 3 details |
|---|
| Sequence | AGGGCCTGCACTCTACAAAC | CATCGACACGAGGCAACCTA | Start: 3310 |
| Tm | 59.963 | 59.827 | End: 3497 |
| Size | 20 | 20 | 188 |
| Primer 4 details |
|---|
| Sequence | TTTCCGTACGCTCTTTCCCC | CATCGACACGAGGCAACCTA | Start: 3380 |
| Tm | 60.037 | 59.827 | End: 3497 |
| Size | 20 | 20 | 118 |
| Primer 5 details |
|---|
| Sequence | TTCGTGTCGCTGTCACCTTT | GGCGATCTCATCCACCTCAG | Start: 3539 |
| Tm | 60.179 | 59.967 | End: 3776 |
| Size | 20 | 20 | 238 |
(T)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTGGTCTGCTTGTGTCTGGA | GGTGCACTACAAAGCAACGG | Start: 905 |
| Tm | 59.893 | 60.04 | End: 1015 |
| Size | 20 | 20 | 111 |
| Primer 2 details |
|---|
| Sequence | GGGCCTGCACTCTACAAACT | CATCGACACGAGGCAACCTA | Start: 3311 |
| Tm | 59.963 | 59.827 | End: 3497 |
| Size | 20 | 20 | 187 |
| Primer 3 details |
|---|
| Sequence | AGGGCCTGCACTCTACAAAC | CATCGACACGAGGCAACCTA | Start: 3310 |
| Tm | 59.963 | 59.827 | End: 3497 |
| Size | 20 | 20 | 188 |
| Primer 4 details |
|---|
| Sequence | TTTCCGTACGCTCTTTCCCC | CATCGACACGAGGCAACCTA | Start: 3380 |
| Tm | 60.037 | 59.827 | End: 3497 |
| Size | 20 | 20 | 118 |
| Primer 5 details |
|---|
| Sequence | TTCGTGTCGCTGTCACCTTT | GGCGATCTCATCCACCTCAG | Start: 3539 |
| Tm | 60.179 | 59.967 | End: 3776 |
| Size | 20 | 20 | 238 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTGGTCTGCTTGTGTCTGGA | GGTGCACTACAAAGCAACGG | Start: 905 |
| Tm | 59.893 | 60.04 | End: 1015 |
| Size | 20 | 20 | 111 |
| Primer 2 details |
|---|
| Sequence | GGGCCTGCACTCTACAAACT | CATCGACACGAGGCAACCTA | Start: 3311 |
| Tm | 59.963 | 59.827 | End: 3497 |
| Size | 20 | 20 | 187 |
| Primer 3 details |
|---|
| Sequence | AGGGCCTGCACTCTACAAAC | CATCGACACGAGGCAACCTA | Start: 3310 |
| Tm | 59.963 | 59.827 | End: 3497 |
| Size | 20 | 20 | 188 |
| Primer 4 details |
|---|
| Sequence | TTTCCGTACGCTCTTTCCCC | CATCGACACGAGGCAACCTA | Start: 3380 |
| Tm | 60.037 | 59.827 | End: 3497 |
| Size | 20 | 20 | 118 |
| Primer 5 details |
|---|
| Sequence | TTCGTGTCGCTGTCACCTTT | GGCGATCTCATCCACCTCAG | Start: 3539 |
| Tm | 60.179 | 59.967 | End: 3776 |
| Size | 20 | 20 | 238 |
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