ssr_primer
Primer Details for TRINITY_DN12997_c3_g5_i1 |
|---|
| (AC)6: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTTCCCCACCCATGACCTTC | CTCTGAGCATCGTGGCTAGG | Start: 465 |
| Tm | 60.034 | 59.968 | End: 688 |
| Size | 20 | 20 | 224 |
| Primer 2 details |
|---|
| Sequence | CTTCCCCACCCATGACCTTC | CCTCTGAGCATCGTGGCTAG | Start: 465 |
| Tm | 60.034 | 59.968 | End: 689 |
| Size | 20 | 20 | 225 |
| Primer 3 details |
|---|
| Sequence | GGTCCAAGTCTTCCTCTGCC | TCAATCCGTCCCCCTTAGGT | Start: 25 |
| Tm | 60.036 | 59.956 | End: 209 |
| Size | 20 | 20 | 185 |
| Primer 4 details |
|---|
| Sequence | ACGTGTTAGCCAAGCCATGA | TCAATCCGTCCCCCTTAGGT | Start: 100 |
| Tm | 59.964 | 59.956 | End: 209 |
| Size | 20 | 20 | 110 |
| Primer 5 details |
|---|
| Sequence | TCTTTCCCTCCTCCACCACA | CTCTGAGCATCGTGGCTAGG | Start: 494 |
| Tm | 60.105 | 59.968 | End: 688 |
| Size | 20 | 20 | 195 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTTCCCCACCCATGACCTTC | CTCTGAGCATCGTGGCTAGG | Start: 465 |
| Tm | 60.034 | 59.968 | End: 688 |
| Size | 20 | 20 | 224 |
| Primer 2 details |
|---|
| Sequence | CTTCCCCACCCATGACCTTC | CCTCTGAGCATCGTGGCTAG | Start: 465 |
| Tm | 60.034 | 59.968 | End: 689 |
| Size | 20 | 20 | 225 |
| Primer 3 details |
|---|
| Sequence | GGTCCAAGTCTTCCTCTGCC | TCAATCCGTCCCCCTTAGGT | Start: 25 |
| Tm | 60.036 | 59.956 | End: 209 |
| Size | 20 | 20 | 185 |
| Primer 4 details |
|---|
| Sequence | ACGTGTTAGCCAAGCCATGA | TCAATCCGTCCCCCTTAGGT | Start: 100 |
| Tm | 59.964 | 59.956 | End: 209 |
| Size | 20 | 20 | 110 |
| Primer 5 details |
|---|
| Sequence | TCTTTCCCTCCTCCACCACA | CTCTGAGCATCGTGGCTAGG | Start: 494 |
| Tm | 60.105 | 59.968 | End: 688 |
| Size | 20 | 20 | 195 |
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