ssr_primer
Primer Details for TRINITY_DN12997_c3_g3_i1 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACGGCTATCACCTCTCACCT | GGGCCGTAGTTATGGACGAG | Start: 218 |
| Tm | 60.033 | 59.968 | End: 461 |
| Size | 20 | 20 | 244 |
| Primer 2 details |
|---|
| Sequence | CTCGTCCATAACTACGGCCC | GTGCTCGACTTCAGGGGAAA | Start: 442 |
| Tm | 59.968 | 59.965 | End: 558 |
| Size | 20 | 20 | 117 |
| Primer 3 details |
|---|
| Sequence | AGATCCACCAGTGCTTCTGC | TGGGAGGATCGATGGAGGTT | Start: 742 |
| Tm | 60.036 | 60.031 | End: 928 |
| Size | 20 | 20 | 187 |
| Primer 4 details |
|---|
| Sequence | CCAACTGCCAACCGTATCCT | AGGTGAGAGGTGATAGCCGT | Start: 15 |
| Tm | 60.035 | 60.033 | End: 237 |
| Size | 20 | 20 | 223 |
| Primer 5 details |
|---|
| Sequence | TTTCCCCTGAAGTCGAGCAC | GCAGAAGCACTGGTGGATCT | Start: 539 |
| Tm | 59.965 | 60.036 | End: 761 |
| Size | 20 | 20 | 223 |
(AT)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACGGCTATCACCTCTCACCT | GGGCCGTAGTTATGGACGAG | Start: 218 |
| Tm | 60.033 | 59.968 | End: 461 |
| Size | 20 | 20 | 244 |
| Primer 2 details |
|---|
| Sequence | CTCGTCCATAACTACGGCCC | GTGCTCGACTTCAGGGGAAA | Start: 442 |
| Tm | 59.968 | 59.965 | End: 558 |
| Size | 20 | 20 | 117 |
| Primer 3 details |
|---|
| Sequence | AGATCCACCAGTGCTTCTGC | TGGGAGGATCGATGGAGGTT | Start: 742 |
| Tm | 60.036 | 60.031 | End: 928 |
| Size | 20 | 20 | 187 |
| Primer 4 details |
|---|
| Sequence | CCAACTGCCAACCGTATCCT | AGGTGAGAGGTGATAGCCGT | Start: 15 |
| Tm | 60.035 | 60.033 | End: 237 |
| Size | 20 | 20 | 223 |
| Primer 5 details |
|---|
| Sequence | TTTCCCCTGAAGTCGAGCAC | GCAGAAGCACTGGTGGATCT | Start: 539 |
| Tm | 59.965 | 60.036 | End: 761 |
| Size | 20 | 20 | 223 |
(A)18: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACGGCTATCACCTCTCACCT | GGGCCGTAGTTATGGACGAG | Start: 218 |
| Tm | 60.033 | 59.968 | End: 461 |
| Size | 20 | 20 | 244 |
| Primer 2 details |
|---|
| Sequence | CTCGTCCATAACTACGGCCC | GTGCTCGACTTCAGGGGAAA | Start: 442 |
| Tm | 59.968 | 59.965 | End: 558 |
| Size | 20 | 20 | 117 |
| Primer 3 details |
|---|
| Sequence | AGATCCACCAGTGCTTCTGC | TGGGAGGATCGATGGAGGTT | Start: 742 |
| Tm | 60.036 | 60.031 | End: 928 |
| Size | 20 | 20 | 187 |
| Primer 4 details |
|---|
| Sequence | CCAACTGCCAACCGTATCCT | AGGTGAGAGGTGATAGCCGT | Start: 15 |
| Tm | 60.035 | 60.033 | End: 237 |
| Size | 20 | 20 | 223 |
| Primer 5 details |
|---|
| Sequence | TTTCCCCTGAAGTCGAGCAC | GCAGAAGCACTGGTGGATCT | Start: 539 |
| Tm | 59.965 | 60.036 | End: 761 |
| Size | 20 | 20 | 223 |
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