ssr_primer
Primer Details for TRINITY_DN12996_c4_g1_i12 |
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| (A)10tcatgaacagtggaattagaaaaaatt(A)18: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTCCATCCATCCCCGAACT | ATTTCGGGCCATGGAACCAT | Start: 3986 |
| Tm | 60.031 | 60.032 | End: 4126 |
| Size | 20 | 20 | 141 |
| Primer 2 details |
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| Sequence | ACTCTCCATCCATCCCCGAA | ATTTCGGGCCATGGAACCAT | Start: 3984 |
| Tm | 60.031 | 60.032 | End: 4126 |
| Size | 20 | 20 | 143 |
| Primer 3 details |
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| Sequence | TATCAGCTTGTGCACCACGT | TTCGGGGATGGATGGAGAGT | Start: 3797 |
| Tm | 59.965 | 60.031 | End: 4003 |
| Size | 20 | 20 | 207 |
| Primer 4 details |
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| Sequence | TATCAGCTTGTGCACCACGT | AGTTCGGGGATGGATGGAGA | Start: 3797 |
| Tm | 59.965 | 60.031 | End: 4005 |
| Size | 20 | 20 | 209 |
| Primer 5 details |
|---|
| Sequence | CCAAGCCATTCAGCTCTGGA | ATTTCGGGCCATGGAACCAT | Start: 3919 |
| Tm | 60.035 | 60.032 | End: 4126 |
| Size | 20 | 20 | 208 |
(AG)6aatgttcgtagcgaaagagcgagcgaaggggagagagaa(AG)7: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTCCATCCATCCCCGAACT | ATTTCGGGCCATGGAACCAT | Start: 3986 |
| Tm | 60.031 | 60.032 | End: 4126 |
| Size | 20 | 20 | 141 |
| Primer 2 details |
|---|
| Sequence | ACTCTCCATCCATCCCCGAA | ATTTCGGGCCATGGAACCAT | Start: 3984 |
| Tm | 60.031 | 60.032 | End: 4126 |
| Size | 20 | 20 | 143 |
| Primer 3 details |
|---|
| Sequence | TATCAGCTTGTGCACCACGT | TTCGGGGATGGATGGAGAGT | Start: 3797 |
| Tm | 59.965 | 60.031 | End: 4003 |
| Size | 20 | 20 | 207 |
| Primer 4 details |
|---|
| Sequence | TATCAGCTTGTGCACCACGT | AGTTCGGGGATGGATGGAGA | Start: 3797 |
| Tm | 59.965 | 60.031 | End: 4005 |
| Size | 20 | 20 | 209 |
| Primer 5 details |
|---|
| Sequence | CCAAGCCATTCAGCTCTGGA | ATTTCGGGCCATGGAACCAT | Start: 3919 |
| Tm | 60.035 | 60.032 | End: 4126 |
| Size | 20 | 20 | 208 |
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