ssr_primers

ssr_primer

Primer Details for TRINITY_DN12991_c2_g5_i4
(TA)6: Primer 1 details
Primer Characteristics	Forward Primer	Reverse Primer	Product Details
Sequence	GGCTGTCCACAATACCACCA	TTCGGCCCATAGCTCGAATC	Start: 5127
Tm	59.962	59.966	End: 5404
Size	20	20	278
Primer 2 details
Sequence	GCCAGGTATCGTCGGCATTA	CATTGATAGTGGCGGCATGC	Start: 5966
Tm	59.967	60.04	End: 6177
Size	20	20	212
Primer 3 details
Sequence	GCCAGGTATCGTCGGCATTA	GCATTGATAGTGGCGGCATG	Start: 5966
Tm	59.967	60.04	End: 6178
Size	20	20	213
Primer 4 details
Sequence	GGCTGTCCACAATACCACCA	GGCCCATAGCTCGAATCTCC	Start: 5127
Tm	59.962	60.037	End: 5401
Size	20	20	275
Primer 5 details
Sequence	ACCGATCCTCTTCCCTCCAA	CCAGTAGTGTTCCATGCGGT	Start: 3586
Tm	59.957	60.036	End: 3722
Size	20	20	137
(A)11: Primer 1 details
Primer Characteristics	Forward Primer	Reverse Primer	Product Details
Sequence	GGCTGTCCACAATACCACCA	TTCGGCCCATAGCTCGAATC	Start: 5127
Tm	59.962	59.966	End: 5404
Size	20	20	278
Primer 2 details
Sequence	GCCAGGTATCGTCGGCATTA	CATTGATAGTGGCGGCATGC	Start: 5966
Tm	59.967	60.04	End: 6177
Size	20	20	212
Primer 3 details
Sequence	GCCAGGTATCGTCGGCATTA	GCATTGATAGTGGCGGCATG	Start: 5966
Tm	59.967	60.04	End: 6178
Size	20	20	213
Primer 4 details
Sequence	GGCTGTCCACAATACCACCA	GGCCCATAGCTCGAATCTCC	Start: 5127
Tm	59.962	60.037	End: 5401
Size	20	20	275
Primer 5 details
Sequence	ACCGATCCTCTTCCCTCCAA	CCAGTAGTGTTCCATGCGGT	Start: 3586
Tm	59.957	60.036	End: 3722
Size	20	20	137
(CT)7: Primer 1 details
Primer Characteristics	Forward Primer	Reverse Primer	Product Details
Sequence	GGCTGTCCACAATACCACCA	TTCGGCCCATAGCTCGAATC	Start: 5127
Tm	59.962	59.966	End: 5404
Size	20	20	278
Primer 2 details
Sequence	GCCAGGTATCGTCGGCATTA	CATTGATAGTGGCGGCATGC	Start: 5966
Tm	59.967	60.04	End: 6177
Size	20	20	212
Primer 3 details
Sequence	GCCAGGTATCGTCGGCATTA	GCATTGATAGTGGCGGCATG	Start: 5966
Tm	59.967	60.04	End: 6178
Size	20	20	213
Primer 4 details
Sequence	GGCTGTCCACAATACCACCA	GGCCCATAGCTCGAATCTCC	Start: 5127
Tm	59.962	60.037	End: 5401
Size	20	20	275
Primer 5 details
Sequence	ACCGATCCTCTTCCCTCCAA	CCAGTAGTGTTCCATGCGGT	Start: 3586
Tm	59.957	60.036	End: 3722
Size	20	20	137
(A)10: Primer 1 details
Primer Characteristics	Forward Primer	Reverse Primer	Product Details
Sequence	GGCTGTCCACAATACCACCA	TTCGGCCCATAGCTCGAATC	Start: 5127
Tm	59.962	59.966	End: 5404
Size	20	20	278
Primer 2 details
Sequence	GCCAGGTATCGTCGGCATTA	CATTGATAGTGGCGGCATGC	Start: 5966
Tm	59.967	60.04	End: 6177
Size	20	20	212
Primer 3 details
Sequence	GCCAGGTATCGTCGGCATTA	GCATTGATAGTGGCGGCATG	Start: 5966
Tm	59.967	60.04	End: 6178
Size	20	20	213
Primer 4 details
Sequence	GGCTGTCCACAATACCACCA	GGCCCATAGCTCGAATCTCC	Start: 5127
Tm	59.962	60.037	End: 5401
Size	20	20	275
Primer 5 details
Sequence	ACCGATCCTCTTCCCTCCAA	CCAGTAGTGTTCCATGCGGT	Start: 3586
Tm	59.957	60.036	End: 3722
Size	20	20	137
(A)12: Primer 1 details
Primer Characteristics	Forward Primer	Reverse Primer	Product Details
Sequence	GGCTGTCCACAATACCACCA	TTCGGCCCATAGCTCGAATC	Start: 5127
Tm	59.962	59.966	End: 5404
Size	20	20	278
Primer 2 details
Sequence	GCCAGGTATCGTCGGCATTA	CATTGATAGTGGCGGCATGC	Start: 5966
Tm	59.967	60.04	End: 6177
Size	20	20	212
Primer 3 details
Sequence	GCCAGGTATCGTCGGCATTA	GCATTGATAGTGGCGGCATG	Start: 5966
Tm	59.967	60.04	End: 6178
Size	20	20	213
Primer 4 details
Sequence	GGCTGTCCACAATACCACCA	GGCCCATAGCTCGAATCTCC	Start: 5127
Tm	59.962	60.037	End: 5401
Size	20	20	275
Primer 5 details
Sequence	ACCGATCCTCTTCCCTCCAA	CCAGTAGTGTTCCATGCGGT	Start: 3586
Tm	59.957	60.036	End: 3722
Size	20	20	137
(T)12: Primer 1 details
Primer Characteristics	Forward Primer	Reverse Primer	Product Details
Sequence	GGCTGTCCACAATACCACCA	TTCGGCCCATAGCTCGAATC	Start: 5127
Tm	59.962	59.966	End: 5404
Size	20	20	278
Primer 2 details
Sequence	GCCAGGTATCGTCGGCATTA	CATTGATAGTGGCGGCATGC	Start: 5966
Tm	59.967	60.04	End: 6177
Size	20	20	212
Primer 3 details
Sequence	GCCAGGTATCGTCGGCATTA	GCATTGATAGTGGCGGCATG	Start: 5966
Tm	59.967	60.04	End: 6178
Size	20	20	213
Primer 4 details
Sequence	GGCTGTCCACAATACCACCA	GGCCCATAGCTCGAATCTCC	Start: 5127
Tm	59.962	60.037	End: 5401
Size	20	20	275
Primer 5 details
Sequence	ACCGATCCTCTTCCCTCCAA	CCAGTAGTGTTCCATGCGGT	Start: 3586
Tm	59.957	60.036	End: 3722
Size	20	20	137
(AGA)5: Primer 1 details
Primer Characteristics	Forward Primer	Reverse Primer	Product Details
Sequence	GGCTGTCCACAATACCACCA	TTCGGCCCATAGCTCGAATC	Start: 5127
Tm	59.962	59.966	End: 5404
Size	20	20	278
Primer 2 details
Sequence	GCCAGGTATCGTCGGCATTA	CATTGATAGTGGCGGCATGC	Start: 5966
Tm	59.967	60.04	End: 6177
Size	20	20	212
Primer 3 details
Sequence	GCCAGGTATCGTCGGCATTA	GCATTGATAGTGGCGGCATG	Start: 5966
Tm	59.967	60.04	End: 6178
Size	20	20	213
Primer 4 details
Sequence	GGCTGTCCACAATACCACCA	GGCCCATAGCTCGAATCTCC	Start: 5127
Tm	59.962	60.037	End: 5401
Size	20	20	275
Primer 5 details
Sequence	ACCGATCCTCTTCCCTCCAA	CCAGTAGTGTTCCATGCGGT	Start: 3586
Tm	59.957	60.036	End: 3722
Size	20	20	137

CsExSLDb: Transcriptome based Cucumis sativus Extended Shelf Life Database

Primer Details for TRINITY_DN12991_c2_g5_i4

ICAR Data Use Licence