ssr_primer
Primer Details for TRINITY_DN12991_c2_g1_i2 |
|---|
| (A)11: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CACCGCCGTGTTTGAGTTTT | AGTCCAACGCATCCTTGGAG | Start: 605 |
| Tm | 59.9 | 60.036 | End: 876 |
| Size | 20 | 20 | 272 |
| Primer 2 details |
|---|
| Sequence | ACACCGAGCTCTCTCTGTCT | TGTCGGTTGTTGGTTTCGGT | Start: 440 |
| Tm | 59.962 | 60.393 | End: 582 |
| Size | 20 | 20 | 143 |
| Primer 3 details |
|---|
| Sequence | CACCGCCGTGTTTGAGTTTT | CAACGCATCCTTGGAGCTCT | Start: 605 |
| Tm | 59.9 | 60.392 | End: 872 |
| Size | 20 | 20 | 268 |
| Primer 4 details |
|---|
| Sequence | CACCGCCGTGTTTGAGTTTT | AAGTCCAACGCATCCTTGGA | Start: 605 |
| Tm | 59.9 | 59.6 | End: 877 |
| Size | 20 | 20 | 273 |
| Primer 5 details |
|---|
| Sequence | CACCGCCGTGTTTGAGTTTT | GCATCCTTGGAGCTCTAGCA | Start: 605 |
| Tm | 59.9 | 59.533 | End: 868 |
| Size | 20 | 20 | 264 |
(T)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CACCGCCGTGTTTGAGTTTT | AGTCCAACGCATCCTTGGAG | Start: 605 |
| Tm | 59.9 | 60.036 | End: 876 |
| Size | 20 | 20 | 272 |
| Primer 2 details |
|---|
| Sequence | ACACCGAGCTCTCTCTGTCT | TGTCGGTTGTTGGTTTCGGT | Start: 440 |
| Tm | 59.962 | 60.393 | End: 582 |
| Size | 20 | 20 | 143 |
| Primer 3 details |
|---|
| Sequence | CACCGCCGTGTTTGAGTTTT | CAACGCATCCTTGGAGCTCT | Start: 605 |
| Tm | 59.9 | 60.392 | End: 872 |
| Size | 20 | 20 | 268 |
| Primer 4 details |
|---|
| Sequence | CACCGCCGTGTTTGAGTTTT | AAGTCCAACGCATCCTTGGA | Start: 605 |
| Tm | 59.9 | 59.6 | End: 877 |
| Size | 20 | 20 | 273 |
| Primer 5 details |
|---|
| Sequence | CACCGCCGTGTTTGAGTTTT | GCATCCTTGGAGCTCTAGCA | Start: 605 |
| Tm | 59.9 | 59.533 | End: 868 |
| Size | 20 | 20 | 264 |
(A)12ccctt(TC)6cctctctccctctctcc(CT)7atatagaaggaatccctgtggcacagttaccaaagttgagagaaatgaatgaaaatgaa(AATG)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CACCGCCGTGTTTGAGTTTT | AGTCCAACGCATCCTTGGAG | Start: 605 |
| Tm | 59.9 | 60.036 | End: 876 |
| Size | 20 | 20 | 272 |
| Primer 2 details |
|---|
| Sequence | ACACCGAGCTCTCTCTGTCT | TGTCGGTTGTTGGTTTCGGT | Start: 440 |
| Tm | 59.962 | 60.393 | End: 582 |
| Size | 20 | 20 | 143 |
| Primer 3 details |
|---|
| Sequence | CACCGCCGTGTTTGAGTTTT | CAACGCATCCTTGGAGCTCT | Start: 605 |
| Tm | 59.9 | 60.392 | End: 872 |
| Size | 20 | 20 | 268 |
| Primer 4 details |
|---|
| Sequence | CACCGCCGTGTTTGAGTTTT | AAGTCCAACGCATCCTTGGA | Start: 605 |
| Tm | 59.9 | 59.6 | End: 877 |
| Size | 20 | 20 | 273 |
| Primer 5 details |
|---|
| Sequence | CACCGCCGTGTTTGAGTTTT | GCATCCTTGGAGCTCTAGCA | Start: 605 |
| Tm | 59.9 | 59.533 | End: 868 |
| Size | 20 | 20 | 264 |
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