ssr_primer
Primer Details for TRINITY_DN12981_c0_g3_i1 |
|---|
| (A)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGGGAAAGGGTAATTGGGGG | CGCCGGATTTTCCAACGATC | Start: 3480 |
| Tm | 60.032 | 59.972 | End: 3710 |
| Size | 20 | 20 | 231 |
| Primer 2 details |
|---|
| Sequence | AAGGCGTAAAGGTGAGTGGG | CGCCGGATTTTCCAACGATC | Start: 3552 |
| Tm | 59.964 | 59.972 | End: 3710 |
| Size | 20 | 20 | 159 |
| Primer 3 details |
|---|
| Sequence | ACGCATGTCCTCCTTCTGTG | CCCCCAATTACCCTTTCCCC | Start: 3307 |
| Tm | 60.037 | 60.032 | End: 3499 |
| Size | 20 | 20 | 193 |
| Primer 4 details |
|---|
| Sequence | ACGCATGTCCTCCTTCTGTG | CCCACTCACCTTTACGCCTT | Start: 3307 |
| Tm | 60.037 | 59.964 | End: 3571 |
| Size | 20 | 20 | 265 |
| Primer 5 details |
|---|
| Sequence | AGGGGTACTTGGACACCACT | ATCAGATGCATGGCCCACAA | Start: 2830 |
| Tm | 60.105 | 60.033 | End: 2991 |
| Size | 20 | 20 | 162 |
(AAG)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGGGAAAGGGTAATTGGGGG | CGCCGGATTTTCCAACGATC | Start: 3480 |
| Tm | 60.032 | 59.972 | End: 3710 |
| Size | 20 | 20 | 231 |
| Primer 2 details |
|---|
| Sequence | AAGGCGTAAAGGTGAGTGGG | CGCCGGATTTTCCAACGATC | Start: 3552 |
| Tm | 59.964 | 59.972 | End: 3710 |
| Size | 20 | 20 | 159 |
| Primer 3 details |
|---|
| Sequence | ACGCATGTCCTCCTTCTGTG | CCCCCAATTACCCTTTCCCC | Start: 3307 |
| Tm | 60.037 | 60.032 | End: 3499 |
| Size | 20 | 20 | 193 |
| Primer 4 details |
|---|
| Sequence | ACGCATGTCCTCCTTCTGTG | CCCACTCACCTTTACGCCTT | Start: 3307 |
| Tm | 60.037 | 59.964 | End: 3571 |
| Size | 20 | 20 | 265 |
| Primer 5 details |
|---|
| Sequence | AGGGGTACTTGGACACCACT | ATCAGATGCATGGCCCACAA | Start: 2830 |
| Tm | 60.105 | 60.033 | End: 2991 |
| Size | 20 | 20 | 162 |
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