ssr_primer
Primer Details for TRINITY_DN12970_c1_g3_i6 |
|---|
| (T)11: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACCGACCGAATCAACAGCTT | ACCCTTCGGATCCGTTGAAC | Start: 945 |
| Tm | 59.965 | 60.037 | End: 1080 |
| Size | 20 | 20 | 136 |
| Primer 2 details |
|---|
| Sequence | ACCGACCGAATCAACAGCTT | CAACCCTTCGGATCCGTTGA | Start: 945 |
| Tm | 59.965 | 60.037 | End: 1082 |
| Size | 20 | 20 | 138 |
| Primer 3 details |
|---|
| Sequence | TAACGATGTGTGTCGGGTGG | AAGCTGTTGATTCGGTCGGT | Start: 717 |
| Tm | 60.038 | 59.965 | End: 964 |
| Size | 20 | 20 | 248 |
| Primer 4 details |
|---|
| Sequence | TTTCTCCTCCGGAACAGCAC | CCACCCGACACACATCGTTA | Start: 596 |
| Tm | 59.965 | 60.038 | End: 736 |
| Size | 20 | 20 | 141 |
| Primer 5 details |
|---|
| Sequence | ACCGACCGAATCAACAGCTT | CTGCAGTAATGATGCGCGAC | Start: 945 |
| Tm | 59.965 | 60.043 | End: 1050 |
| Size | 20 | 20 | 106 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACCGACCGAATCAACAGCTT | ACCCTTCGGATCCGTTGAAC | Start: 945 |
| Tm | 59.965 | 60.037 | End: 1080 |
| Size | 20 | 20 | 136 |
| Primer 2 details |
|---|
| Sequence | ACCGACCGAATCAACAGCTT | CAACCCTTCGGATCCGTTGA | Start: 945 |
| Tm | 59.965 | 60.037 | End: 1082 |
| Size | 20 | 20 | 138 |
| Primer 3 details |
|---|
| Sequence | TAACGATGTGTGTCGGGTGG | AAGCTGTTGATTCGGTCGGT | Start: 717 |
| Tm | 60.038 | 59.965 | End: 964 |
| Size | 20 | 20 | 248 |
| Primer 4 details |
|---|
| Sequence | TTTCTCCTCCGGAACAGCAC | CCACCCGACACACATCGTTA | Start: 596 |
| Tm | 59.965 | 60.038 | End: 736 |
| Size | 20 | 20 | 141 |
| Primer 5 details |
|---|
| Sequence | ACCGACCGAATCAACAGCTT | CTGCAGTAATGATGCGCGAC | Start: 945 |
| Tm | 59.965 | 60.043 | End: 1050 |
| Size | 20 | 20 | 106 |
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