ssr_primer
Primer Details for TRINITY_DN12970_c1_g1_i15 |
|---|
| (A)15: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTGGCAAAAGGAAAGCAGGG | GCAACAACGGGTTCTGGTTC | Start: 4555 |
| Tm | 59.966 | 59.97 | End: 4834 |
| Size | 20 | 20 | 280 |
| Primer 2 details |
|---|
| Sequence | ACGACGGTGCTGCTCTAAAA | CGATGATGGTGGTGGTGGAA | Start: 6956 |
| Tm | 59.967 | 60.036 | End: 7080 |
| Size | 20 | 20 | 125 |
| Primer 3 details |
|---|
| Sequence | CCAGGCACACCCGAATAGTT | CCCTGCTTTCCTTTTGCCAC | Start: 4371 |
| Tm | 60.035 | 59.966 | End: 4574 |
| Size | 20 | 20 | 204 |
| Primer 4 details |
|---|
| Sequence | TTTGCATTTTGAAGCCCCGG | AACTATTCGGGTGTGCCTGG | Start: 4152 |
| Tm | 59.965 | 60.035 | End: 4390 |
| Size | 20 | 20 | 239 |
| Primer 5 details |
|---|
| Sequence | TGTACCTGACCGGGCTTTTC | CGATGATGGTGGTGGTGGAA | Start: 6897 |
| Tm | 59.964 | 60.036 | End: 7080 |
| Size | 20 | 20 | 184 |
(T)11caaatcaataaaatttgagggctaacccatttttatatgtgccaaacatatacaactacaccctctttttccttcactgcac(AT)6: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTGGCAAAAGGAAAGCAGGG | GCAACAACGGGTTCTGGTTC | Start: 4555 |
| Tm | 59.966 | 59.97 | End: 4834 |
| Size | 20 | 20 | 280 |
| Primer 2 details |
|---|
| Sequence | ACGACGGTGCTGCTCTAAAA | CGATGATGGTGGTGGTGGAA | Start: 6956 |
| Tm | 59.967 | 60.036 | End: 7080 |
| Size | 20 | 20 | 125 |
| Primer 3 details |
|---|
| Sequence | CCAGGCACACCCGAATAGTT | CCCTGCTTTCCTTTTGCCAC | Start: 4371 |
| Tm | 60.035 | 59.966 | End: 4574 |
| Size | 20 | 20 | 204 |
| Primer 4 details |
|---|
| Sequence | TTTGCATTTTGAAGCCCCGG | AACTATTCGGGTGTGCCTGG | Start: 4152 |
| Tm | 59.965 | 60.035 | End: 4390 |
| Size | 20 | 20 | 239 |
| Primer 5 details |
|---|
| Sequence | TGTACCTGACCGGGCTTTTC | CGATGATGGTGGTGGTGGAA | Start: 6897 |
| Tm | 59.964 | 60.036 | End: 7080 |
| Size | 20 | 20 | 184 |
(GCA)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTGGCAAAAGGAAAGCAGGG | GCAACAACGGGTTCTGGTTC | Start: 4555 |
| Tm | 59.966 | 59.97 | End: 4834 |
| Size | 20 | 20 | 280 |
| Primer 2 details |
|---|
| Sequence | ACGACGGTGCTGCTCTAAAA | CGATGATGGTGGTGGTGGAA | Start: 6956 |
| Tm | 59.967 | 60.036 | End: 7080 |
| Size | 20 | 20 | 125 |
| Primer 3 details |
|---|
| Sequence | CCAGGCACACCCGAATAGTT | CCCTGCTTTCCTTTTGCCAC | Start: 4371 |
| Tm | 60.035 | 59.966 | End: 4574 |
| Size | 20 | 20 | 204 |
| Primer 4 details |
|---|
| Sequence | TTTGCATTTTGAAGCCCCGG | AACTATTCGGGTGTGCCTGG | Start: 4152 |
| Tm | 59.965 | 60.035 | End: 4390 |
| Size | 20 | 20 | 239 |
| Primer 5 details |
|---|
| Sequence | TGTACCTGACCGGGCTTTTC | CGATGATGGTGGTGGTGGAA | Start: 6897 |
| Tm | 59.964 | 60.036 | End: 7080 |
| Size | 20 | 20 | 184 |
(GCA)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTGGCAAAAGGAAAGCAGGG | GCAACAACGGGTTCTGGTTC | Start: 4555 |
| Tm | 59.966 | 59.97 | End: 4834 |
| Size | 20 | 20 | 280 |
| Primer 2 details |
|---|
| Sequence | ACGACGGTGCTGCTCTAAAA | CGATGATGGTGGTGGTGGAA | Start: 6956 |
| Tm | 59.967 | 60.036 | End: 7080 |
| Size | 20 | 20 | 125 |
| Primer 3 details |
|---|
| Sequence | CCAGGCACACCCGAATAGTT | CCCTGCTTTCCTTTTGCCAC | Start: 4371 |
| Tm | 60.035 | 59.966 | End: 4574 |
| Size | 20 | 20 | 204 |
| Primer 4 details |
|---|
| Sequence | TTTGCATTTTGAAGCCCCGG | AACTATTCGGGTGTGCCTGG | Start: 4152 |
| Tm | 59.965 | 60.035 | End: 4390 |
| Size | 20 | 20 | 239 |
| Primer 5 details |
|---|
| Sequence | TGTACCTGACCGGGCTTTTC | CGATGATGGTGGTGGTGGAA | Start: 6897 |
| Tm | 59.964 | 60.036 | End: 7080 |
| Size | 20 | 20 | 184 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTGGCAAAAGGAAAGCAGGG | GCAACAACGGGTTCTGGTTC | Start: 4555 |
| Tm | 59.966 | 59.97 | End: 4834 |
| Size | 20 | 20 | 280 |
| Primer 2 details |
|---|
| Sequence | ACGACGGTGCTGCTCTAAAA | CGATGATGGTGGTGGTGGAA | Start: 6956 |
| Tm | 59.967 | 60.036 | End: 7080 |
| Size | 20 | 20 | 125 |
| Primer 3 details |
|---|
| Sequence | CCAGGCACACCCGAATAGTT | CCCTGCTTTCCTTTTGCCAC | Start: 4371 |
| Tm | 60.035 | 59.966 | End: 4574 |
| Size | 20 | 20 | 204 |
| Primer 4 details |
|---|
| Sequence | TTTGCATTTTGAAGCCCCGG | AACTATTCGGGTGTGCCTGG | Start: 4152 |
| Tm | 59.965 | 60.035 | End: 4390 |
| Size | 20 | 20 | 239 |
| Primer 5 details |
|---|
| Sequence | TGTACCTGACCGGGCTTTTC | CGATGATGGTGGTGGTGGAA | Start: 6897 |
| Tm | 59.964 | 60.036 | End: 7080 |
| Size | 20 | 20 | 184 |
(TC)6: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTGGCAAAAGGAAAGCAGGG | GCAACAACGGGTTCTGGTTC | Start: 4555 |
| Tm | 59.966 | 59.97 | End: 4834 |
| Size | 20 | 20 | 280 |
| Primer 2 details |
|---|
| Sequence | ACGACGGTGCTGCTCTAAAA | CGATGATGGTGGTGGTGGAA | Start: 6956 |
| Tm | 59.967 | 60.036 | End: 7080 |
| Size | 20 | 20 | 125 |
| Primer 3 details |
|---|
| Sequence | CCAGGCACACCCGAATAGTT | CCCTGCTTTCCTTTTGCCAC | Start: 4371 |
| Tm | 60.035 | 59.966 | End: 4574 |
| Size | 20 | 20 | 204 |
| Primer 4 details |
|---|
| Sequence | TTTGCATTTTGAAGCCCCGG | AACTATTCGGGTGTGCCTGG | Start: 4152 |
| Tm | 59.965 | 60.035 | End: 4390 |
| Size | 20 | 20 | 239 |
| Primer 5 details |
|---|
| Sequence | TGTACCTGACCGGGCTTTTC | CGATGATGGTGGTGGTGGAA | Start: 6897 |
| Tm | 59.964 | 60.036 | End: 7080 |
| Size | 20 | 20 | 184 |
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