ssr_primer
Primer Details for TRINITY_DN12931_c1_g1_i1 |
|---|
| (A)12: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTGCGGTCATCAAACGATGG | AAAGTGCGGGAAGATGACGT | Start: 181 |
| Tm | 59.903 | 59.965 | End: 389 |
| Size | 20 | 20 | 209 |
| Primer 2 details |
|---|
| Sequence | TCCTTCGATTTTTGCGCCAC | AAAGTGCGGGAAGATGACGT | Start: 259 |
| Tm | 59.76 | 59.965 | End: 389 |
| Size | 20 | 20 | 131 |
| Primer 3 details |
|---|
| Sequence | CCTTCGATTTTTGCGCCACT | AAAGTGCGGGAAGATGACGT | Start: 260 |
| Tm | 59.759 | 59.965 | End: 389 |
| Size | 20 | 20 | 130 |
| Primer 4 details |
|---|
| Sequence | GTGCGGTCATCAAACGATGG | AGTCAAAAGGGAAGTGGGCC | Start: 181 |
| Tm | 59.903 | 60.179 | End: 353 |
| Size | 20 | 20 | 173 |
| Primer 5 details |
|---|
| Sequence | CGTGCGGTCATCAAACGATG | AAAGTGCGGGAAGATGACGT | Start: 180 |
| Tm | 60.247 | 59.965 | End: 389 |
| Size | 20 | 20 | 210 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTGCGGTCATCAAACGATGG | AAAGTGCGGGAAGATGACGT | Start: 181 |
| Tm | 59.903 | 59.965 | End: 389 |
| Size | 20 | 20 | 209 |
| Primer 2 details |
|---|
| Sequence | TCCTTCGATTTTTGCGCCAC | AAAGTGCGGGAAGATGACGT | Start: 259 |
| Tm | 59.76 | 59.965 | End: 389 |
| Size | 20 | 20 | 131 |
| Primer 3 details |
|---|
| Sequence | CCTTCGATTTTTGCGCCACT | AAAGTGCGGGAAGATGACGT | Start: 260 |
| Tm | 59.759 | 59.965 | End: 389 |
| Size | 20 | 20 | 130 |
| Primer 4 details |
|---|
| Sequence | GTGCGGTCATCAAACGATGG | AGTCAAAAGGGAAGTGGGCC | Start: 181 |
| Tm | 59.903 | 60.179 | End: 353 |
| Size | 20 | 20 | 173 |
| Primer 5 details |
|---|
| Sequence | CGTGCGGTCATCAAACGATG | AAAGTGCGGGAAGATGACGT | Start: 180 |
| Tm | 60.247 | 59.965 | End: 389 |
| Size | 20 | 20 | 210 |
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