ssr_primer
Primer Details for TRINITY_DN12930_c4_g1_i11 |
|---|
| (T)16: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATGGGAAGCTCGAATTGGCA | GGGTTTGGTGGACGGTTAGT | Start: 816 |
| Tm | 60.034 | 59.891 | End: 1025 |
| Size | 20 | 20 | 210 |
| Primer 2 details |
|---|
| Sequence | ATGGGAAGCTCGAATTGGCA | GTTAGGGTTTGGTGGACGGT | Start: 816 |
| Tm | 60.034 | 59.891 | End: 1029 |
| Size | 20 | 20 | 214 |
| Primer 3 details |
|---|
| Sequence | ACCGTCCACCAAACCCTAAC | CCGATCAGATTCCGACGGAG | Start: 1010 |
| Tm | 59.891 | 60.04 | End: 1254 |
| Size | 20 | 20 | 245 |
| Primer 4 details |
|---|
| Sequence | ACTAACCGTCCACCAAACCC | CCGATCAGATTCCGACGGAG | Start: 1006 |
| Tm | 59.891 | 60.04 | End: 1254 |
| Size | 20 | 20 | 249 |
| Primer 5 details |
|---|
| Sequence | TTTCCCGTCACAATGCTCCA | CCGATCAGATTCCGACGGAG | Start: 1080 |
| Tm | 59.89 | 60.04 | End: 1254 |
| Size | 20 | 20 | 175 |
(T)13: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATGGGAAGCTCGAATTGGCA | GGGTTTGGTGGACGGTTAGT | Start: 816 |
| Tm | 60.034 | 59.891 | End: 1025 |
| Size | 20 | 20 | 210 |
| Primer 2 details |
|---|
| Sequence | ATGGGAAGCTCGAATTGGCA | GTTAGGGTTTGGTGGACGGT | Start: 816 |
| Tm | 60.034 | 59.891 | End: 1029 |
| Size | 20 | 20 | 214 |
| Primer 3 details |
|---|
| Sequence | ACCGTCCACCAAACCCTAAC | CCGATCAGATTCCGACGGAG | Start: 1010 |
| Tm | 59.891 | 60.04 | End: 1254 |
| Size | 20 | 20 | 245 |
| Primer 4 details |
|---|
| Sequence | ACTAACCGTCCACCAAACCC | CCGATCAGATTCCGACGGAG | Start: 1006 |
| Tm | 59.891 | 60.04 | End: 1254 |
| Size | 20 | 20 | 249 |
| Primer 5 details |
|---|
| Sequence | TTTCCCGTCACAATGCTCCA | CCGATCAGATTCCGACGGAG | Start: 1080 |
| Tm | 59.89 | 60.04 | End: 1254 |
| Size | 20 | 20 | 175 |
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