ssr_primer
Primer Details for TRINITY_DN12930_c3_g2_i1 |
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| (A)23: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGTCGTGGGTATGAATGCC | GCTTTGCCCCAAGGTGAAAG | Start: 278 |
| Tm | 59.82 | 59.966 | End: 481 |
| Size | 20 | 20 | 204 |
| Primer 2 details |
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| Sequence | AGGTCGTGGGTATGAATGCC | TGATGGAATGGGTGTTGGGG | Start: 278 |
| Tm | 59.82 | 59.959 | End: 424 |
| Size | 20 | 20 | 147 |
| Primer 3 details |
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| Sequence | CAGGGGCAGTAGCTTGCTTA | GCTTTGCCCCAAGGTGAAAG | Start: 342 |
| Tm | 59.747 | 59.966 | End: 481 |
| Size | 20 | 20 | 140 |
| Primer 4 details |
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| Sequence | GGGCAGTAGCTTGCTTACCT | GCTTTGCCCCAAGGTGAAAG | Start: 345 |
| Tm | 59.747 | 59.966 | End: 481 |
| Size | 20 | 20 | 137 |
| Primer 5 details |
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| Sequence | AGGGGCAGTAGCTTGCTTAC | GCTTTGCCCCAAGGTGAAAG | Start: 343 |
| Tm | 59.747 | 59.966 | End: 481 |
| Size | 20 | 20 | 139 |
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