ssr_primer
Primer Details for TRINITY_DN12916_c1_g3_i4 |
|---|
| (CT)7: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAACTTGTTCGCGTGTCACG | TCAATTGGGATGCTGGGGTC | Start: 1872 |
| Tm | 59.974 | 60.033 | End: 1975 |
| Size | 20 | 20 | 104 |
| Primer 2 details |
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| Sequence | ATCCGCTCTTCACTCCCTCT | CCTGGGAGCGAAAGAGATCC | Start: 30 |
| Tm | 60.033 | 59.894 | End: 300 |
| Size | 20 | 20 | 271 |
| Primer 3 details |
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| Sequence | GATGGGAGGTCTTGTTGGCA | ACTCCACCGACGCCAATAAG | Start: 670 |
| Tm | 59.962 | 60.108 | End: 946 |
| Size | 20 | 20 | 277 |
| Primer 4 details |
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| Sequence | GAGGGTTCCCTCGACCATTG | GCAGCCTGTGTTTAGCCAAC | Start: 1007 |
| Tm | 60.108 | 60.039 | End: 1259 |
| Size | 20 | 20 | 253 |
| Primer 5 details |
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| Sequence | ATCCGCTCTTCACTCCCTCT | AGGAGTCGAAGGAAGACCCA | Start: 30 |
| Tm | 60.033 | 59.885 | End: 255 |
| Size | 20 | 20 | 226 |
(TTC)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAACTTGTTCGCGTGTCACG | TCAATTGGGATGCTGGGGTC | Start: 1872 |
| Tm | 59.974 | 60.033 | End: 1975 |
| Size | 20 | 20 | 104 |
| Primer 2 details |
|---|
| Sequence | ATCCGCTCTTCACTCCCTCT | CCTGGGAGCGAAAGAGATCC | Start: 30 |
| Tm | 60.033 | 59.894 | End: 300 |
| Size | 20 | 20 | 271 |
| Primer 3 details |
|---|
| Sequence | GATGGGAGGTCTTGTTGGCA | ACTCCACCGACGCCAATAAG | Start: 670 |
| Tm | 59.962 | 60.108 | End: 946 |
| Size | 20 | 20 | 277 |
| Primer 4 details |
|---|
| Sequence | GAGGGTTCCCTCGACCATTG | GCAGCCTGTGTTTAGCCAAC | Start: 1007 |
| Tm | 60.108 | 60.039 | End: 1259 |
| Size | 20 | 20 | 253 |
| Primer 5 details |
|---|
| Sequence | ATCCGCTCTTCACTCCCTCT | AGGAGTCGAAGGAAGACCCA | Start: 30 |
| Tm | 60.033 | 59.885 | End: 255 |
| Size | 20 | 20 | 226 |
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