ssr_primer
Primer Details for TRINITY_DN12912_c2_g2_i21 |
|---|
| (T)25: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGTGAGTTTCAGCATACGCG | CTCCATCGCCATACCTGCAT | Start: 834 |
| Tm | 59.976 | 59.964 | End: 965 |
| Size | 20 | 20 | 132 |
| Primer 2 details |
|---|
| Sequence | ATGCAGGTATGGCGATGGAG | GTTGCCAAGGCCAAAGACTG | Start: 946 |
| Tm | 59.964 | 59.967 | End: 1147 |
| Size | 20 | 20 | 202 |
| Primer 3 details |
|---|
| Sequence | CGTGAGTTTCAGCATACGCG | CGGCATTATCAGTGGGCTCT | Start: 834 |
| Tm | 59.976 | 59.893 | End: 983 |
| Size | 20 | 20 | 150 |
| Primer 4 details |
|---|
| Sequence | AGAGCCCACTGATAATGCCG | GTTGCCAAGGCCAAAGACTG | Start: 964 |
| Tm | 59.893 | 59.967 | End: 1147 |
| Size | 20 | 20 | 184 |
| Primer 5 details |
|---|
| Sequence | GAGCTGCTTGAGACTCAGGG | TACAAGGCGCTTACCACCTG | Start: 1311 |
| Tm | 60.108 | 60.037 | End: 1501 |
| Size | 20 | 20 | 191 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGTGAGTTTCAGCATACGCG | CTCCATCGCCATACCTGCAT | Start: 834 |
| Tm | 59.976 | 59.964 | End: 965 |
| Size | 20 | 20 | 132 |
| Primer 2 details |
|---|
| Sequence | ATGCAGGTATGGCGATGGAG | GTTGCCAAGGCCAAAGACTG | Start: 946 |
| Tm | 59.964 | 59.967 | End: 1147 |
| Size | 20 | 20 | 202 |
| Primer 3 details |
|---|
| Sequence | CGTGAGTTTCAGCATACGCG | CGGCATTATCAGTGGGCTCT | Start: 834 |
| Tm | 59.976 | 59.893 | End: 983 |
| Size | 20 | 20 | 150 |
| Primer 4 details |
|---|
| Sequence | AGAGCCCACTGATAATGCCG | GTTGCCAAGGCCAAAGACTG | Start: 964 |
| Tm | 59.893 | 59.967 | End: 1147 |
| Size | 20 | 20 | 184 |
| Primer 5 details |
|---|
| Sequence | GAGCTGCTTGAGACTCAGGG | TACAAGGCGCTTACCACCTG | Start: 1311 |
| Tm | 60.108 | 60.037 | End: 1501 |
| Size | 20 | 20 | 191 |
(CT)8cactcactcactcacaattctgtttactaattcgcttgtcttcccaattggattcgttgtcactgtaaacatactttgtg(CT)10cactcactcacaattctgtttactaattcgcttgtcttcccaattggattcgttgtcactgtaaacatactttgtg(CT)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGTGAGTTTCAGCATACGCG | CTCCATCGCCATACCTGCAT | Start: 834 |
| Tm | 59.976 | 59.964 | End: 965 |
| Size | 20 | 20 | 132 |
| Primer 2 details |
|---|
| Sequence | ATGCAGGTATGGCGATGGAG | GTTGCCAAGGCCAAAGACTG | Start: 946 |
| Tm | 59.964 | 59.967 | End: 1147 |
| Size | 20 | 20 | 202 |
| Primer 3 details |
|---|
| Sequence | CGTGAGTTTCAGCATACGCG | CGGCATTATCAGTGGGCTCT | Start: 834 |
| Tm | 59.976 | 59.893 | End: 983 |
| Size | 20 | 20 | 150 |
| Primer 4 details |
|---|
| Sequence | AGAGCCCACTGATAATGCCG | GTTGCCAAGGCCAAAGACTG | Start: 964 |
| Tm | 59.893 | 59.967 | End: 1147 |
| Size | 20 | 20 | 184 |
| Primer 5 details |
|---|
| Sequence | GAGCTGCTTGAGACTCAGGG | TACAAGGCGCTTACCACCTG | Start: 1311 |
| Tm | 60.108 | 60.037 | End: 1501 |
| Size | 20 | 20 | 191 |
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