ssr_primer
Primer Details for TRINITY_DN12907_c5_g7_i2 |
|---|
| (A)13: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TACATGGTGGCTTGCCAGAG | AGCTTGCTGACCGTTGATGA | Start: 465 |
| Tm | 60.035 | 59.965 | End: 727 |
| Size | 20 | 20 | 263 |
| Primer 2 details |
|---|
| Sequence | TCATCAACGGTCAGCAAGCT | GCCATTGGAACCAAGCAGTG | Start: 708 |
| Tm | 59.965 | 60.038 | End: 962 |
| Size | 20 | 20 | 255 |
| Primer 3 details |
|---|
| Sequence | GGAGTTACCAGCACAGCAGT | GCCATTGGAACCAAGCAGTG | Start: 743 |
| Tm | 59.965 | 60.038 | End: 962 |
| Size | 20 | 20 | 220 |
| Primer 4 details |
|---|
| Sequence | GTCTTTGAAAGGCACCAGCG | ACCGTGCAAGTCCGAGAAAT | Start: 2358 |
| Tm | 60.04 | 59.965 | End: 2524 |
| Size | 20 | 20 | 167 |
| Primer 5 details |
|---|
| Sequence | CGTCTTTGAAAGGCACCAGC | ACCGTGCAAGTCCGAGAAAT | Start: 2357 |
| Tm | 60.04 | 59.965 | End: 2524 |
| Size | 20 | 20 | 168 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TACATGGTGGCTTGCCAGAG | AGCTTGCTGACCGTTGATGA | Start: 465 |
| Tm | 60.035 | 59.965 | End: 727 |
| Size | 20 | 20 | 263 |
| Primer 2 details |
|---|
| Sequence | TCATCAACGGTCAGCAAGCT | GCCATTGGAACCAAGCAGTG | Start: 708 |
| Tm | 59.965 | 60.038 | End: 962 |
| Size | 20 | 20 | 255 |
| Primer 3 details |
|---|
| Sequence | GGAGTTACCAGCACAGCAGT | GCCATTGGAACCAAGCAGTG | Start: 743 |
| Tm | 59.965 | 60.038 | End: 962 |
| Size | 20 | 20 | 220 |
| Primer 4 details |
|---|
| Sequence | GTCTTTGAAAGGCACCAGCG | ACCGTGCAAGTCCGAGAAAT | Start: 2358 |
| Tm | 60.04 | 59.965 | End: 2524 |
| Size | 20 | 20 | 167 |
| Primer 5 details |
|---|
| Sequence | CGTCTTTGAAAGGCACCAGC | ACCGTGCAAGTCCGAGAAAT | Start: 2357 |
| Tm | 60.04 | 59.965 | End: 2524 |
| Size | 20 | 20 | 168 |
(AGA)13cgatttagggtttgaagaacacaaaagcaaatcaaaatcccaggattc(A)12cactttatatagaac(GA)6: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TACATGGTGGCTTGCCAGAG | AGCTTGCTGACCGTTGATGA | Start: 465 |
| Tm | 60.035 | 59.965 | End: 727 |
| Size | 20 | 20 | 263 |
| Primer 2 details |
|---|
| Sequence | TCATCAACGGTCAGCAAGCT | GCCATTGGAACCAAGCAGTG | Start: 708 |
| Tm | 59.965 | 60.038 | End: 962 |
| Size | 20 | 20 | 255 |
| Primer 3 details |
|---|
| Sequence | GGAGTTACCAGCACAGCAGT | GCCATTGGAACCAAGCAGTG | Start: 743 |
| Tm | 59.965 | 60.038 | End: 962 |
| Size | 20 | 20 | 220 |
| Primer 4 details |
|---|
| Sequence | GTCTTTGAAAGGCACCAGCG | ACCGTGCAAGTCCGAGAAAT | Start: 2358 |
| Tm | 60.04 | 59.965 | End: 2524 |
| Size | 20 | 20 | 167 |
| Primer 5 details |
|---|
| Sequence | CGTCTTTGAAAGGCACCAGC | ACCGTGCAAGTCCGAGAAAT | Start: 2357 |
| Tm | 60.04 | 59.965 | End: 2524 |
| Size | 20 | 20 | 168 |
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