Primer Details for TRINITY_DN12889_c8_g1_i1 |
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| (A)21: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTTGAACCTGGGAGGCAGAG | TCTCTTCTTTCCTATCCTAACTTCTT | Start: 5 |
| Tm | 60.036 | 57.097 | End: 134 |
| Size | 20 | 26 | 130 |
| Primer 2 details |
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| Sequence | AACCTGGGAGGCAGAGTTTG | TCTCTTCTTTCCTATCCTAACTTCTT | Start: 9 |
| Tm | 59.889 | 57.097 | End: 134 |
| Size | 20 | 26 | 126 |
| Primer 3 details |
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| Sequence | GAACCTGGGAGGCAGAGTTT | TCTCTTCTTTCCTATCCTAACTTCTT | Start: 8 |
| Tm | 59.598 | 57.097 | End: 134 |
| Size | 20 | 26 | 127 |
| Primer 4 details |
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| Sequence | CTTGAACCTGGGAGGCAGAG | TCTCTTCTTTCCTATCCTAACTTCTTT | Start: 5 |
| Tm | 60.036 | 57.672 | End: 134 |
| Size | 20 | 27 | 130 |
| Primer 5 details |
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| Sequence | AACCTGGGAGGCAGAGTTTG | TCTCTTCTTTCCTATCCTAACTTCTTT | Start: 9 |
| Tm | 59.889 | 57.672 | End: 134 |
| Size | 20 | 27 | 126 |
